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Influence of dietary phosphorus on mineralization and morphology of Indian major carp, catla (Catla catla) fingerlings

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Abstract

The present study was conducted to investigate the changes in mineralization and morphology in response to graded levels of dietary phosphorus in Indian major carp, Catla (Catla catla) fingerlings (av. wt. 4.23 ± 0.87 g). The experimental system constituted of 24 150-L capacity plastic tanks (eight treatments, three replicates) stocked with 20 fingerlings each. Eight isonitrogenous and isocaloric semi-purified diets (crude protein 35%, crude lipid 8.5%) were formulated with graded levels of phosphorus (0%-control; 0.1; 0.3; 0.5; 0.7; 0.9; 1.1; 1.3; 1.5%) using KH2PO4. The fish were fed twice daily with the respective feeds for a period of 120 days. The percentage of ash content in whole body, vertebrae and opercular bones was found to be significantly (P < 0.05) lower in fish fed low levels of dietary phosphorus (T1, T2) in comparison with other treatments. The concentration of phosphorus and calcium was found to be significantly (P < 0.05) lower in whole body, vertebrae and opercula of fish fed low phosphorus diets (T1, T2) relative to treatments with higher levels of dietary phosphorus supplementation. The concentration of magnesium in opercular and vertebral tissues of T1 group was significantly (P < 0.05) lower than all other groups. The X-ray radiographs did not reveal any skeletal deformities in any of the treatments. Truss analysis showed absence of significant morphological variations between fish of different treatments. The deficiency of dietary phosphorus in catla led to significantly lower mineralization of whole body and bone tissues, but the deficiency was not severe enough to cause skeletal deformities and morphological changes in the fish.

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Abbreviations

M trans :

Transformed truss distances

M :

Original truss distances

SL :

Standard length of fish

SL mean :

Overall mean standard length

β :

Within group slope regressions of the log M against log SL

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Acknowledgments

The scholarship received by the first author from the Central Institute of Fisheries Education (CIFE), Mumbai, for the PhD study is gratefully acknowledged. The authors are thankful to the Director, CIFE, for providing all the facilities required for the work. The authors gratefully acknowledge the technical assistance received from Mr. A. K. Padmanabhan, Technical officer, AEMD division, CIFE and Ms. Vijula K PhD scholar, AEMD division, CIFE for the estimation of minerals using AAS.

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Authors and Affiliations

  1. Central Institute of Brackishwater Aquaculture, 75 Santhome High Road, Raja Annamalai Puram, Chennai, Tamil Nadu, 600 028, India

    Krishna Sukumaran

  2. Fish Nutrition, Biochemistry and Physiology Division, Central Institute of Fisheries Education, Fisheries University Road, Versova, Mumbai, 400 061, India

    Asim K. Pal & Narottam P. Sahu

  3. Fish Genetics and Biotechnology Division, Central Institute of Fisheries Education, Fisheries University Road, Versova, Mumbai, 400 061, India

    Shrinivas Jahageerdar

  4. Department of Zoology, University of Oklahoma, 730 Van Vleet, Oval, OK, 73019, USA

    Deepak Khambadakone

  5. Central Inland Fisheries Research Institute, Regional Centre, HOUSEFED Complex, Dispur, Guwahati, Assam, 781 006, India

    Dipesh Debnath

Authors
  1. Krishna Sukumaran
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  2. Asim K. Pal
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  3. Narottam P. Sahu
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  4. Shrinivas Jahageerdar
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  5. Deepak Khambadakone
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  6. Dipesh Debnath
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Correspondence to Dipesh Debnath.

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Sukumaran, K., Pal, A.K., Sahu, N.P. et al. Influence of dietary phosphorus on mineralization and morphology of Indian major carp, catla (Catla catla) fingerlings. Aquacult Int 19, 749–763 (2011). https://doi.org/10.1007/s10499-010-9392-x

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  • DOI: https://doi.org/10.1007/s10499-010-9392-x

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