Skip to main content
Log in

Antibacterial activity of Allium sativum against multidrug-resistant Vibrio harveyi isolated from black gill–diseased Fenneropenaeus indicus

  • Published:
Aquaculture International Aims and scope Submit manuscript

Abstract

The present study evaluated the in vitro and in vivo inhibitory effects of Allium sativum extracts against the multidrug-resistant Vibrio harveyi, isolated from the black gill–diseased Fenneropenaeus indicus. Initially, the antibiogram of pathogenic V. harveyi was documented for its resistance towards selected commercial antibiotics. Further, antibacterial activity of A. sativum against Vibrio harveyi was performed by the agar well diffusion method. V. harveyi culture was seeded over the agar plates, and each of the garlic extracts (freshly squeezed garlic extract—FSGE, freeze-dried garlic extract—FDGE and methanolic garlic extract—MGE) was tested at three different concentrations. FSGE showed greater inhibitory activity against V. harveyi than FDGE and MGE. Three diets of standard meal preparation for shrimp containing 0% garlic as a control, 0.5% garlic and 1% garlic treatments were formulated to evaluate the effects of garlic on the experimental infection of shrimp with V. harveyi. The combined results of long- and short-term garlic (1%) extract treatment showed a 75% reduction in cumulative mortality. The present study discusses the advantages of using freshly squeezed garlic extract treatment in shrimp culture that could offer a promising alternative to the use of antibiotics in aquaculture to control disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Ankri S, Mirelman D (1999) Antimicrobial properties of allicin from garlic. Microbes Infect 2:125–129

    Article  Google Scholar 

  • Ankri S, Miron T, Rabinkov A, Wilchek M, Mirelman D et al (1997) Allicin from garlic strongly inhibits cystein proteinases and cytopathic effects of Entamoeba histolytica. Antimicrob Agents Chemother 10:2286–2288

    Google Scholar 

  • Arora SD, Kaur L (1999) Antimicrobial activity of spices. Int J Antimicrob Agents 12:257–262

    Article  PubMed  CAS  Google Scholar 

  • Austin B, Zhang XH (2006) Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Lett Appl Microbiol 43:119–124

    Article  PubMed  CAS  Google Scholar 

  • Bauer RW, Kirby MDK, Sherris JC, Turck M et al (1966) Antibiotic susceptibility testing by standard single disc diffusion method. Am J Clin Pathol 45:493–496

    PubMed  CAS  Google Scholar 

  • Block E (1985) The chemistry of garlic and onions. Sci Am 252:114–119

    Article  PubMed  CAS  Google Scholar 

  • Cowman MM (1999) Plant products as antimicrobial agents. Clin Microbiol Rev 12:564–582

    Google Scholar 

  • Gatesoupe FJ (1991) Siderophore production and probiotics effect of Vibrio spp. associated with turbot larvae, Scophthalmus maximus. Aquat Liv Res 10:239–246

    Article  Google Scholar 

  • Gatesoupe FJ (2000) The use of probiotics in aquaculture. Aquaculture 180:147–165

    Article  Google Scholar 

  • Gibbons S (2005) Plants as a source of bacterial resistance modulators and anti-infectious agents. Phyto Rev 4:63–78

    Article  CAS  Google Scholar 

  • Harzevili ARS, Vanduffel H, Dhert P, Swings J, Sorgeloos P et al (1998) Use of a potential probiotic Lactococcus lactis AR21 strain for the enhancement of growth in the rotifer Brachionus plicatilis (Muller). Aqua Res 29:411–417

    Google Scholar 

  • Holt JG, Krieg NR, Sneath PHA, Staley JT (1994) Facultatively anaerobic Gram negative rods. In: Hensy WR (ed) Bergey’s manual of determinative bacteriology, 9th edn. Williams & Wilkins, Baltimore, pp 175–289

    Google Scholar 

  • Indu MN, Hatha AAM, Abirosh C, Harsha U, Vivekanandan G et al (2006) Antimicrobial activity of some of the south-Indian spices against serotypes of Escherichia coli, Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Braz J Microbiol 37:153–158

    Article  Google Scholar 

  • Karunasagar I (1994) Mass mortality of Penaeus monodon larvae to antibiotic resistant Vibrio harveyi infection. Aquaculture 128:03–209

    Article  Google Scholar 

  • Koch HP, Lawson LD (eds) (1996) Garlic: the science and therapeutic application of Allium sativum L. and related species, 2nd edn. William and Wilkins, Baltimore, p 329

  • Lundin GG (1996) Fish health and quarantine. In: Global attempts to address shrimp diseases. Marine/Environmental Paper No. 4. Land, water and Natural Habitats Division, Environment Department, World Bank, Rome, p 45

  • Palavesam A, Sheeja L, Immanuel G et al (2006) Antimicrobial properties of medicinal herbal extracts against pathogenic bacteria isolated from the infected grouper Epinephelus tauvina. J Biol Res 6:167–176

    Google Scholar 

  • Pang L, Zhang XH, Zhong Y, Chen J, Li J, Austin B et al (2006) Identification of Vibrio harveyi using PCR amplification of the toxR gene. Lett Appl Microbiol 43:249–255

    Article  PubMed  CAS  Google Scholar 

  • Ramasamy P (ed) (1995) Diseases of shrimps in aquaculture systems; diagnosis and therapeutic measures. Vanitha publications, Madras

    Google Scholar 

  • Rengpipat S, Phianphak W, Piyatiratitivorakul S, Menasveta P et al (1998) Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture 167:301–313

    Article  Google Scholar 

  • Richard EK, Anthony TT (eds) (1983) Plant and fungal toxins. Marcel Dekker, New york

    Google Scholar 

  • Ruangpan L, Kitao T (1991) Vibrio bacteria isolated from black tiger shrimp Penaeus monodon Fabricius. J Fish Dis 14:383–388

    Article  Google Scholar 

  • Sokmen A, Jonesand BM, Erturk M et al (1999) The in vitro antibacterial activity of Turkish plants. J Ethnopharmacol 67:79–86

    Article  PubMed  CAS  Google Scholar 

  • Subhasinghe R, Bartly DM, Megladdery S, Barg U (1998) Sustainable shrimp culture development: biotechnological issues and challenges. In: Flegel TW et al (eds) Advances in shrimp biotechnology. National centre for genetic engineering and Biotechnology, Bangkok, pp 13–18

    Google Scholar 

  • Ushimaru PI, Nogueira da Silva MT, Claudio Di Stasi L, Barbosa L, Junior AF et al (2007) Antibacterial activity of medicinal plant extracts. Braz J Microbiol 38:717–719

    Article  Google Scholar 

  • Vaseeharan B, Ramasamy P (2003) Abundance of potentially pathogenic microorganisms in Penaeus monodon larvae rearing systems in India. Microbiol Res 158:299–308

    Article  PubMed  Google Scholar 

  • Vaseeharan B, Lin J, Ramasamy P et al (2004) Effect of probiotics, antibiotic sensitivity, pathogenicity and plasmid profiles of Listonella anguillarum-like bacteria isolated from Penaeus monodon culture systems. Aquaculture 241:77–91

    Article  CAS  Google Scholar 

  • Vuddhakul V, Bhoopong P, Hayeebilan F, Subhadhirasaku S et al (2007) Inhibitory activity of Thai condiments on pandemic strain of Vibrio parahaemolyticus. Food Microbiol 24:413–418

    Article  PubMed  Google Scholar 

  • YanaYano Y, Satomi M, Oikawa H et al (2006) Antimicrobial effect of spices and herbs on Vibrio parahaemolyticus. Int J Food Microbial 111:6–11

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by University Grants Commission (UGC), India. The grants through Indo-Taiwan programme of cooperation in Science and Technology from Department of Science and Technology, New Delhi, India, and National Science Council, Taiwan are gratefully acknowledged.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to B. Vaseeharan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vaseeharan, B., Prasad, G.S., Ramasamy, P. et al. Antibacterial activity of Allium sativum against multidrug-resistant Vibrio harveyi isolated from black gill–diseased Fenneropenaeus indicus . Aquacult Int 19, 531–539 (2011). https://doi.org/10.1007/s10499-010-9369-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10499-010-9369-9

Keywords

Navigation