Abstract
Turbot Scophthalmus maximus exhibits sexually dimorphic growth, with females growing faster and reaching larger adult sizes than males. Thus, development of techniques for preferentially producing females is necessary to optimize production of these species. In this paper, gynogenetic diploids of turbot were induced by activating egg development with ultraviolet (UV)-irradiated left-eyed flounder Paralichthys olivaceus sperm combined with cold shock to prevent extrusion of the second polar body. The results of UV irradiation experiments showed that survival, motility, and duration of activity of P. olivaceus sperm generally decreased with increase in UV dose. The typical Hertwig’s effect was observed after fertilized turbot eggs with UV-irradiated P. olivaceus sperm and the optimal UV dose for gynogenetic haploid production was 36,000 erg mm−2. At 15°C, appropriate timing of cold shock for retention of the second polar body in turbot eggs was at 6 min after fertilization. Results of different combinations of two shock temperatures (1 or 3°C) and four shock durations (15, 25, 35 or 45 min) at 6 min after fertilization demonstrated that shock of 25 min at 1°C gave the highest production of diploid gynogens (39.58% relative to its diploid control). The results of this study reveal that the use of UV-irradiated P. olivaceus sperm for activation of turbot eggs and cold shock for polar body retention is an effective method to produce gynogenetic offspring.
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Acknowledgments
This study was supported by grants from the National Natural Science Foundation of China (30571445), the National 863 High Technology Research Foundation of China (2006AA10A404), Open-End Funds of Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology (2007HS001), and the Natural Science Foundation of Huai Hai Institute of Technology (KX06061). The authors greatly appreciate the assistance of Yang Xuesong and Sun Shouke of DaHua fish farm of Lai Zhou.
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Xu, JH., You, F., Sun, W. et al. Induction of diploid gynogenesis in turbot Scophthalmus maximus with left-eyed flounder Paralichthys olivaceus sperm. Aquacult Int 16, 623–634 (2008). https://doi.org/10.1007/s10499-008-9173-y
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DOI: https://doi.org/10.1007/s10499-008-9173-y