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IRF-1-inhibited lncRNA XIST regulated the osteogenic differentiation via miR-450b/FBXW7 axis

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Abstract

Osteoporosis influences life quality among elder people. Osteoblast dysfunction could cause the occurrence of osteoporosis. LncRNA XIST are involved in the progression of osteoporosis. However, the correlation between IRF-1 and XIST in osteogenic differentiation remains unclear. In the study, Clinical samples were collected for the analysis of XIST level. mRNA and protein levels were detected by RT-qPCR and western blot, respectively. H&E staining was performed to observe the histological changes in mice. Alizarin Red Staining was applied to assess the calcium deposits in hBMSCs. Meanwhile, the relation among XIST, miR-450b and FBXW7 was investigated by dual luciferase assay and ChIP. In vivo model was constructed to assess the impact of XIST in osteoporosis. XIST was found to be upregulated in osteoporosis, and XIST overexpression could inhibit the osteogenic differentiation in hBMSCs. IRF-1 could transcriptionally inhibit the expression of XIST, and XIST could inhibit osteogenic differentiation through binding with miR-450b in hBMSCs. In addition, miR-450b significantly promoted the osteogenic differentiation in hBMSCs via targeting FBXW7. Furthermore, XIST knockdown could inhibit the symptom of osteoporosis in vivo. IRF-1 promoted the osteogenic differentiation via mediation of lncRNA XIST/miR-450b/FBXW7 axis, and this finding might shed novel insights on exploring new ideas against osteoporosis.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

Abbreviations

XIST:

X-inactive-specific transcript

FBXW7:

F-box and WD repeat domain-containing 7

IRF-1:

interferon regulatory factor-1

BMSC:

bone marrow mesenchymal stem cell

OVX:

ovariectomy

OM:

osteogenic medium

BMP2:

bone morphogenetic protein-2

OCN:

osteocalcin

OPN:

osteopontin

RUNX2:

RUNX Family Transcription Factor 2

References

  1. Shin J, Kweon HJ, Kwon KJ, Han SH (2021) Incidence of osteoporosis and ambient air pollution in South Korea: a population-based retrospective cohort study. BMC Public Health 21(1):1794

    Article  PubMed  PubMed Central  Google Scholar 

  2. Ji X, Li Z, Wang W, Chen J (2021) Downregulation of long non-coding RNA PVT1 enhances fracture healing via regulating microRNA-497-5p/HMGA2 axis.Bioengineered.

  3. Chen S, Liu D, Zhou Z, Qin S (2021) Role of long non-coding RNA H19 in the development of osteoporosis. Mol Med 27(1):122

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Pan B, Sun J, Liu Z, Wang L, Huo H, Zhao Y et al (2021) Longxuetongluo Capsule protects against cerebral ischemia/reperfusion injury through endoplasmic reticulum stress and MAPK-mediated mechanisms. J Adv Res 33:215–225

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Bai Y, Liu X, Chen Q, Chen T, Jiang N, Guo Z (2021) Myricetin ameliorates ox-LDL-induced HUVECs apoptosis and inflammation via lncRNA GAS5 upregulating the expression of miR-29a-3p. Sci Rep 11(1):19637

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Li Z, Xue H, Tan G, Xu Z (2021) Effects of miRNAs, lncRNAs and circRNAs on osteoporosis as regulatory factors of bone homeostasis (review).Mol Med Rep. ; 24(5)

  7. Cheng G, Li Y, Liu Z, Song X (2021) lncRNA PSMA3-AS1 promotes the progression of non-small cell lung cancer through targeting miR-17-5p/PD-L1.Adv Clin Exp Med.

  8. Dong J, Xia R, Zhang Z, Xu C (2021) lncRNA MEG3 aggravated neuropathic pain and astrocyte overaction through mediating miR-130a-5p/CXCL12/CXCR4 axis. Aging (Albany NY). ;13(undefined)

  9. Yu J, Xiao M, Ren G (2021) Long non-coding RNA XIST promotes osteoporosis by inhibiting the differentiation of bone marrow mesenchymal stem cell by sponging miR-29b-3p that suppresses nicotinamide N-methyltransferase. Bioengineered 12(1):6057–6069

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Rong W, Wan N, Zheng X, Shi G, Jiang C, Pan K et al (2021) Chrysin inhibits hepatocellular carcinoma progression through suppressing programmed death ligand 1 expression. Phytomedicine 95:153867

    Article  PubMed  Google Scholar 

  11. Shao Y, Hu X, Wu X (2021) LncRNA X inactive-specific transcript promotes osteoclast differentiation through Tgif2 by acting as a ceRNA of mir-590-3p in a murine model. Regen Med 16(7):643–653

    Article  CAS  PubMed  Google Scholar 

  12. Li X (2021) LncRNA MALAT1 promotes osteogenic differentiation of BMSCs and inhibits osteoclastic differentiation of Mo in osteoporosis via the miR-124-3p/IGF2BP1/Wnt/beta-catenin axis.J Tissue Eng Regen Med.

  13. Peng S, Gao Y, Shi S, Zhao D, Cao H, Fu T et al (2021) LncRNA-AK137033 inhibits the osteogenic potential of adipose-derived stem cells in diabetic osteoporosis by regulating wnt signaling pathway via DNA methylation.Cell Prolif. :e13174

  14. Gao W, Zhang Y (2021) Depression of lncRNA MINCR antagonizes LPS-evoked acute injury and inflammatory response via miR-146b-5p and the TRAF6-NFkB signaling. Mol Med 27(1):124

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Jia T, Wang M, Yan W, Wu W, Shen R (2021) Upregulation of mir-489-3p attenuates cerebral ischemia/reperfusion injury by targeting histone deacetylase 2 (HDAC2).Neuroscience.

  16. Yuan Y, Jiang X, Tang L, Wang J, Liu Q, Zou X et al (2021) SNX20AR/MiRNA-301a-3p/SNX20 Axis Associated with Cell Proliferation and Immune Infiltration in Lung Adenocarcinoma. Front Mol Biosci 8:744363

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Ren L, Guo L, Kou N, Lv J, Wang Z, Yang K (2021) LncRNA LINC00963 promotes osteogenic differentiation of hBMSCs and alleviates osteoporosis progression by targeting miRNA-760/ETS1 axis. Autoimmunity 54(6):313–325

    Article  CAS  PubMed  Google Scholar 

  18. Fan L, Fan J, Liu Y, Li T, Xu H, Yang Y et al (2018) miR-450b promotes osteogenic differentiation in Vitro and enhances bone formation in vivo by targeting BMP3. Stem Cells Dev 27(9):600–611

    Article  CAS  PubMed  Google Scholar 

  19. Zhang J, Jia G, Xue P, Li Z (2021) Melatonin restores osteoporosis-impaired osteogenic potential of bone marrow mesenchymal stem cells and alleviates bone loss through the HGF/PTEN/Wnt/beta-catenin axis. Ther Adv Chronic Dis 12:2040622321995685

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Xu C, Shi H, Jiang X, Fan Y, Huang D, Qi X et al (2021) ZEB1 mediates bone marrow mesenchymal stem cell osteogenic differentiation partly via Wnt/beta-Catenin signaling. Front Mol Biosci 8:682728

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Dai J, Xu S, Okada T, Liu Y, Zuo G, Tang J et al (2021) T0901317, an agonist of liver X receptors, attenuates neuronal apoptosis in Early Brain Injury after Subarachnoid hemorrhage in rats via liver X Receptors/Interferon Regulatory Factor/P53 upregulated modulator of Apoptosis/Dynamin-1-Like protein pathway. Oxid Med Cell Longev 2021:8849131

    Article  PubMed  PubMed Central  Google Scholar 

  22. Tong D, Zou E, Bai L, Ma J, Guo N, Wang H et al (2020) IRF-1 mediated long non-coding RNA PVT1-214 promotes oxaliplatin resistance of colorectal cancer via miR-128 inhibition. J BUON 25(5):2205–2214

    PubMed  Google Scholar 

  23. Jiang L, Wan C, Guo Q, Wu J, Cai J (2020) Efficacy of 125I seed implantation combined with intermittent hormonal therapy on moderate- and high-risk non-metastatic prostate cancer. J BUON 25(6):2616–2622

    PubMed  Google Scholar 

  24. Lee KS, Lee J, Kim HK, Yeom SH, Woo CH, Jung YJ et al (2021) Extracellular vesicles from adipose tissue-derived stem cells alleviate osteoporosis through osteoprotegerin and miR-21-5p. J Extracell Vesicles 10(12):e12152

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Lin CM, Wang BW, Pan CM, Fang WJ, Chua SK, Cheng WP et al (2021) Chrysin boosts KLF2 expression through suppression of endothelial cell-derived exosomal microRNA-92a in the model of atheroprotection. Eur J Nutr 60(8):4345–4355

    Article  CAS  PubMed  Google Scholar 

  26. Xiao Y, Liu L, Zheng Y, Liu W, Xu Y (2021) Kaempferol attenuates the effects of XIST/miR-130a/STAT3 on inflammation and extracellular matrix degradation in osteoarthritis. Future Med Chem 13(17):1451–1464

    Article  CAS  PubMed  Google Scholar 

  27. Kuno I, Takayanagi D, Asami Y, Murakami N, Matsuda M, Shimada Y et al (2021) TP53 mutants and non-HPV16/18 genotypes are poor prognostic factors for concurrent chemoradiotherapy in locally advanced cervical cancer. Sci Rep 11(1):19261

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Xu X, Cui Y, Li C, Wang Y, Cheng J, Chen S et al (2021) SETD3 downregulation mediates PTEN Upregulation-Induced ischemic neuronal death through suppression of actin polymerization and mitochondrial function. Mol Neurobiol 58(10):4906–4920

    Article  CAS  PubMed  Google Scholar 

  29. Li Y, Wang J, Ma Y, Du W, Feng K, Wang S (2021) Mir-101-loaded exosomes secreted by bone marrow mesenchymal stem cells requires the FBXW7/HIF1alpha/FOXP3 axis, facilitating osteogenic differentiation. J Cell Physiol 236(6):4258–4272

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

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Funding

This work was sponsored by Fujian provincial health technology project (No.2021CXA013)

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Authors and Affiliations

  1. Department of orthopedics, Fujian Medical University Union Hospital, No.29 Xin quan Road, Gulou District, 350001, Fuzhou, Fujian, P.R. China

    Chenyang Song, Yu Guo, Fenyong Chen & Wenge Liu

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  1. Chenyang Song
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  2. Yu Guo
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  3. Fenyong Chen
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  4. Wenge Liu
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Contributions

Conception of manuscript: EIP working group (all authors).

Manuscript design and writing: Chenyang Song, Yu Guo, Fenyong Chen.

Manuscript reviewing and editing: all authors.

All authors read and agreed to the final version of the manuscript.

Corresponding author

Correspondence to Wenge Liu.

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Ethics approval and consent to participate

These studies were approved by Fujian Medical University Union Hospital Animal Ethics and Experimental Safety Committee.

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The informed consent was obtained from study participants.

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Song, C., Guo, Y., Chen, F. et al. IRF-1-inhibited lncRNA XIST regulated the osteogenic differentiation via miR-450b/FBXW7 axis. Apoptosis 28, 669–680 (2023). https://doi.org/10.1007/s10495-023-01820-w

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  • DOI: https://doi.org/10.1007/s10495-023-01820-w

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