Abstract
The diversity of the bacterial community in the gut is closely related to human health. Gut microbes accomplish multiple physiological and biochemical functions. Sitosterols are a series of phytochemicals that have multiple pharmacological activities and are used as cholesterol-lowering drugs in clinical practice. In this study, we investigated the roles of bacteria and short-chain fatty acids (SCFAs) to the anti-colorectal cancer (anti-CRC) effects of sitosterols in BALB/c nude mice. Sitosterols were administered orally and gut microbiota composition and intestinal SCFAs changes were analyzed. The correlation between gut microbiota, SCFAs, and tumor apoptosis was assessed by a series of in vivo and in vitro experiments. Tumor growth in the mice was inhibited by sitosterol-treatment. Mechanistic studies revealed that sitosterol-treatment reduced the expression of PI3K/Akt, promoted the activation of Bad, decreased Bcl-xl, and enhanced cyto-c release, leading to caspase-9 and caspase-3 activation, PARP cleavage, and apoptosis. 16S rDNA analysis revealed that the diversity of microbiota, particularly phyla Bacteroidetes and Firmicutes, reduced dramatically in the gut of tumor-bearing mice, whilst treatment with sitosterols reversed these changes. The levels of SCFAs in the fecal samples of sitosterol-treated mice increased, leading to cancer cell apoptosis in vitro. Moreover, tumor apoptosis was induced after mice received a daily dose of 2 × 108 CFU/0.2 mL Lactobacillus pentosus or 20 mM/0.2 mL SCFAs. Taken together, these results demonstrate that sitosterols maintain a diverse microbial environment and enrich the content of L. pentosus in the gut, leading to the production of beneficial metabolites including SCFAs that promote tumor apoptosis.
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Abbreviations
- CRC:
-
Colorectal cancer
- SCFAs:
-
Short-chain fatty acids
- NC:
-
Normal control
- TC:
-
Tumor control
- ST:
-
β-Sitosterol
- STG:
-
β-Sitosterol-glucoside
- STGL:
-
β-Sitosterol-glucoside-linoleate
- LAC:
-
Lactobacillus pentosus
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Acknowledgements
This work was financially supported by the Special Program for Scientific and Technical Innovation of Chongqing Social Livelihood (cstc2015shmszx80012, cstc2017shms-kjfp80004), Fundamental Research Funds for the Central Universities (XDJK2017D151, XDJK2017D160), Chongqing Postgraduate Research and Research Innovation Project (CYS17068), Achievement Transfer Program of Institutions of Higher Education in Chongqing (KJZH17105), and County-University Cooperation Innovation Funds of Southwest University (Zx201601).
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Ma, H., Yu, Y., Wang, M. et al. Correlation between microbes and colorectal cancer: tumor apoptosis is induced by sitosterols through promoting gut microbiota to produce short-chain fatty acids. Apoptosis 24, 168–183 (2019). https://doi.org/10.1007/s10495-018-1500-9
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DOI: https://doi.org/10.1007/s10495-018-1500-9