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Apoptosis

, Volume 22, Issue 11, pp 1353–1361 | Cite as

Remote ischemic preconditioning protects human neural stem cells from oxidative stress

  • Ayako Motomura
  • Mikiko Shimizu
  • Akira Kato
  • Kazuya Motomura
  • Akane Yamamichi
  • Hiroko Koyama
  • Fumiharu Ohka
  • Tomohide Nishikawa
  • Yusuke Nishimura
  • Masahito Hara
  • Tetsuya Fukuda
  • Yasuhiko Bando
  • Toshihide Nishimura
  • Toshihiko Wakabayashi
  • Atsushi Natsume
Short Communication

Abstract

In previous clinical trials, we showed that remote ischemic preconditioning (rIPC) reduced myocardial damage in children undergoing treatment for congenital heart defects and postoperative renal failure in patients undergoing abdominal aortic aneurysm surgery. In rabbit experiments, pre-treatment with plasma and plasma dialysate (obtained using 15-kDa cut-off dialysis membrane) from donor rabbits subjected to rIPC similarly protected against cardiac infarction. However, the protective substances containing in rIPC plasma have been unknown. In the present study, we showed that rIPC plasma exerted anti-apoptotic and anti-oxidative effects on human neural stem cells under oxygen glucose deprivation (OGD) that mimics brain ischemia. Additionally, we applied the sample to the liquid chromatography integrated with mass spectrometry to identify candidate key molecules in the rIPC plasma and determine its role in protecting neural stem cells from OGD-induced cell death. Thioredoxin increased significantly after rIPC compared to pre-IPC. Pretreatment with thioredoxin, the antioxidant protein, markedly protected human neural stem cells from OGD-induced cell death. The effect of thioredoxin on brain ischemia in animals should be further evaluated. However, the present study first evaluated the effect of rIPC in the ischemic cellular model.

Keywords

Remote ischemic preconditioning Oxygen glucose deprivation (OGD) Neural stem cells Oxidative stress 

Supplementary material

10495_2017_1425_MOESM1_ESM.pptx (1.1 mb)
Supplementary material 1 (PPTX 1134 KB)

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Ayako Motomura
    • 1
  • Mikiko Shimizu
    • 2
  • Akira Kato
    • 1
  • Kazuya Motomura
    • 1
  • Akane Yamamichi
    • 1
  • Hiroko Koyama
    • 3
  • Fumiharu Ohka
    • 1
  • Tomohide Nishikawa
    • 1
  • Yusuke Nishimura
    • 1
  • Masahito Hara
    • 1
  • Tetsuya Fukuda
    • 4
  • Yasuhiko Bando
    • 4
  • Toshihide Nishimura
    • 5
    • 6
  • Toshihiko Wakabayashi
    • 1
  • Atsushi Natsume
    • 1
  1. 1.Department of NeurosurgeryNagoya University School of MedicineNagoyaJapan
  2. 2.Pediatric Cardiology, The Heart Institute of JapanTokyo Women’s Medical UniversityTokyoJapan
  3. 3.Division of Regeneration and Advanced Medical ScienceGifu University Graduate School of MedicineGifuJapan
  4. 4.Biosys Technologies, Inc.TokyoJapan
  5. 5.Department of Translational Medicine InformaticsSt. Marianna University School of MedicineKanagawaJapan
  6. 6.Center of Excellence in Biological and Medical Mass SpectrometryLund UniversityLundSweden

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