The induction of apoptosis and autophagy in human hepatoma SMMC-7721 cells by combined treatment with vitamin C and polysaccharides extracted from Grifola frondosa
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Polysaccharides extracted from the mushroom Grifola frondosa (GFP) are a potential anticancer agent. The objective of this study was to investigate the effect of GFP and vitamin C (VC) alone and in combination on the viability of human hepatocarcinoma SMMC-7721 and HepG2 cells. Studies designed to detect cell apoptosis and autophagy were also conducted to investigate the mechanism. Results from the cell viability assay indicated that a combination of GFP (0.2 or 0.25 mg/mL) and VC (0.3 mmol/L) (GFP/VC) led to 52.73 and 53.93% reduction in cell viability of SMMC-7721 and HepG2 cells separately after 24 h. Flow cytometric analysis indicated that GFP/VC treatment induced cell cycle arrest at the G2/M phase, and apoptosis occurred in approximately 43.62 and 42.46% of the SMMC-7721 and HepG2 cells separately. Moreover, results of Hoechst33258 and monodansylcadaverine staining, and transmission electron microscopy, showed that GFP/VC induced apoptosis and autophagy in SMMC-7721 and HepG2 cells. Western blot analysis showed changes in the expression of apoptosis-related proteins [upregulation of BAX and caspase-3, downregulation of Bcl-2, and activation of poly-(ADP-ribose)-polymerase] and autophagy protein markers (upregulation of beclin-1 and microtubule-associated protein 1A/1B light chain-3). We also demonstrated that the expression of both Akt and p-Akt was enhanced, suggesting the PI3K/Akt/mTOR pathway might not be involved in this process. Our study shows that the combined application of GFP and VC induced cell apoptosis and autophagy in vitro, and might have antitumor activity in vivo.
KeywordsAnticancer Apoptosis Autophagy Grifola frondosa Vitamin C Hepatoma
Polysaccharides from Grifola frondosa
50% inhibitory concentration
Dulbecco’s Modified Eagle’s Medium
Microtubule-associated protein 1 light chain 3
Phosphate buffered saline
This work was supported by the Fundamental Research Funds for the Central Universities from Northwest Minzu University, China (Nos. 31920140070, 31920160009), The Science and Technology Project of Lanzhou City, China (No. 2016-3-37). And The National Natural Science Foundation of China (31560254; 81260442).
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest.
- 14.Leon IE, Di Virgilio AL, Porro V, Muglia CI, Naso LG, Williams PA, Bollati-Fogolin M, Etcheverry SB (2013) Antitumor properties of a vanadyl (IV) complex with the flavonoid chrysin [VO(chrysin)2 EtOH]2 in a human osteosarcoma model: the role of oxidative stress and apoptosis. Dalton Trans 42:11868–11880CrossRefPubMedGoogle Scholar
- 15.Rajnikumar S, Sudharsan PT, Rajneesh P, Ahmad S, Kutiyanawalla A, Kolhe R, Bhattacharyya MH, Chutkan N, Hunter M, Hill WD, Hamrick M, Isales C, Fulzele S (2015) The crucial role of vitamin C and its transporter (SVCT2) in bone marrow stromal cell autophagy and apoptosis. Stem Cell Res 15:312–321CrossRefGoogle Scholar
- 18.Shi S, Wang Q, Xu J, Jang JH, Padilla MT, Nyunoya T, Xing C, Zhang L, Lin Y (2015) Synergistic anticancer effect of cisplatin and Chal-24 combination through IAP and c-FLIPL degradation, Ripoptosome formation and autophagy-mediated apoptosis. Oncotarget 6:1640–1651CrossRefPubMedPubMedCentralGoogle Scholar
- 25.Wang J, Xiong X, Xing Y, Liu Z, Jiang W, Huang J, Feng B (2013) Chinese herbal medicine for acute mountain sickness: a systematic review of randomized controlled trials. Evid Based Complement Altern Med 2013:732562Google Scholar
- 33.Kroemer G, Galluzzi L, Vandenabeele P, Abrams J, Alnemri ES, Baehrecke EH, Blagosklonny MV, El-Deiry WS, Golstein P, Green DR, Hengartner M, Knight RA, Kumar S, Lipton SA, Malorni W, Nuñez G, Peter ME, Tschopp J, Yuan J, Piacentini M, Zhivotovsky B, Melino G (2009) Classification of cell death: recommendations of the Nomenclature Committee on Cell Death 2009. Cell Death Differ 16:3–11CrossRefPubMedGoogle Scholar
- 39.Aoki H, Takada Y, Kondo S, Sawaya R, Aggarwal BB, Kondo Y (2007) Evidence that curcumin suppresses the growth of malignant gliomas in vitro and in vivo through induction of autophagy: role of Akt and extracellular signal-regulated kinase signaling pathways. Mol Pharmacol 72:29–39CrossRefPubMedGoogle Scholar