, Volume 22, Issue 11, pp 1454–1460 | Cite as

Protodioscin ameliorates oxidative stress, inflammation and histology outcome in Complete Freund’s adjuvant induced arthritis rats

  • Jia-Yu Liu
  • Ya-Ling Hou
  • Rong Cao
  • Hong Xia Qiu
  • Guo-Hua Cheng
  • Ran Tu
  • Li Wang
  • Jun-Li Zhang
  • Dan Liu
Original Paper


Protective effect of protodioscin or methyl protodioscin against inflammation had been reported in various inflammation diseases. This study aimed to investigate the effect of protodioscin against Complete Freund’s adjuvant (CFA)-induced arthritis rats. Rats randomly divided into model groups were injected with CFA, companied with different dose of protodioscin (50, 100, and 200 mg/kg body weight). The histology, changes in biochemical parameters and inflammatory cytokines expression were detected for anti-inflammation effect evaluation of protodioscin. CFA treatment induced arthritic rats with swelling paw, ankle inflammation, and area of lymphocyte infiltration, upregulated inflammatory cytokines (IL-1β, TNF-α, cyclo-oxygenase 2, and IL-6 as well as prostaglandin E2), articular elastase, myeloperoxidase, lipid peroxidase and nitrite oxide levels, downregulated glutathione, catalase, and superoxide dismutase. In contrast, protodioscin ameliorated all the changes induced by CFA in rats, suggesting the anti-inflammatory effect of protodioscin. We concluded that protodioscin administration into CFA-induced arthritis rats protected against CFA-induced oxidative stress, neutrophil infiltration, and inflammation, suggesting the anti-inflammatory effect and the therapeutic potential of protodioscin for arthritis.


Rheumatoid arthritis Protodioscin Inflammatory cytokines Oxidative stress 



This study is supported by Natural Science Foundation of Shaanxi (2015jm8483); Shaanxi Provincial Administration of traditional Chinese medicine research projects (15-JC025) Xi’an Health Science and Techology Project (j2013015, j201603041).

Compliance with Ethical Standards

Conflict of interest

The author(s) declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Jia-Yu Liu
    • 1
    • 2
  • Ya-Ling Hou
    • 1
  • Rong Cao
    • 1
  • Hong Xia Qiu
    • 1
  • Guo-Hua Cheng
    • 1
  • Ran Tu
    • 3
  • Li Wang
    • 1
    • 2
  • Jun-Li Zhang
    • 1
    • 2
  • Dan Liu
    • 1
    • 2
  1. 1.Department of Rheumatology and ImmunologyXi’an No. 5 HospitalXi’anChina
  2. 2.Xi’an Institute of RheumatologyXi’anChina
  3. 3.College of Life SciencesShaanxi Normal UniversityXi’anChina

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