Study on the apoptosis mediated by cytochrome c and factors that affect the activation of bovine longissimus muscle during postmortem aging
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This study investigates whether bovine longissimus muscle cell apoptosis occurs during postmortem aging and whether apoptosis is dependent on the mitochondria pathway. This study also determines the apoptosis process mediated by cytochrome c after its release from mitochondria and the factors that affect the activation processes. Results indicate that apoptotic nuclei were detected at 12 h postmortem. Cytochrome c release from the mitochondria to the cytoplasm activated the caspase-9 and caspase-3 at early postmortem aging and the activation of caspase-9 occurs before the activation of caspase-3. The pH level decreased during the first 48 h postmortem, whereas the mitochondria membrane permeability increased from 6 to 12 h. Results demonstrate that an apoptosis process of bovine muscle occurred during postmortem aging. Apoptosis was dependent on the mitochondria pathway and occurred at early postmortem aging. Increased mitochondria membrane permeability and low pH are necessary conditions for the release of cytochrome c during postmortem aging.
KeywordsApoptosis Cytochrome c-mediated Mitochondria Bovine longissimus muscle Postmortem aging
We thank colleagues in the laboratory and our collaborators for their useful suggestions. This work was supported by the China Agriculture Research System (CARS-38), National Natural Science Foundation of China (Grant No.: 31560463).
Compliance with ethical standards
Conflict of interest
Jiaying Zhang, Qunli Yu, Ling Han, Cheng Chen, Hang Li and Guangxing Han declares that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the author.
Informed consent was obtained from all individual participants included in the study.
- 14.Tassy C, Herrera-Mendez CH, Sentandreu MA, Aubry L, Bremaud L, Pelissier P et al (2005) Muscle endopin 1, amuscle intracellular serpin which strongly inhibits elastase: purification, characterization, cellular localization and tissue distribution. Biochem J 388:273–280CrossRefPubMedPubMedCentralGoogle Scholar
- 17.Qian T, Nieminen AL, Herman B (1997) Mitochondrial permeability transition in pH-dependent reperfusion injury to rat hepatocytes. Am J Physiol 273:1783–1792Google Scholar
- 18.Boudjellal A, Becila S, Coulis G, Herrera-Mendez CH, Aubry L, Lepetit J et al (2008) Is the pH drop profile curvilinear and either monophasic or polyphasic? Consequences on the ultimate bovine meat texture. Afr J Agric Res 3:195–204Google Scholar
- 25.Deng XL, Wang Y, Chou J, Cadet JL (2001) Methamphetamine causes widespread apoptosis in the mouse brain: exidence from using as improved TUNEL histochemical method. Mol Brain Res 93:64–69Google Scholar
- 38.Petrosillo G, Ruggiero FM, Pistolese M, Paradies G (2001) Reactive oxygen species generated from the mitochondrial electron transport chain induce cytochrome c dissociation from beef-heart submitochondrial particles via cardiolipin peroxidation. Possible role in the apoptosis. FEBS Lett 509:435–438CrossRefPubMedGoogle Scholar
- 39.Hofer T, Servais S, Seo AY, Marzetti E, Hiona A, Upadhyay SJ et al (2009) Bioenergetics and permeability transition pore opening in heart subsarcolemmal and interfibrillar mitochondria: effects of aging and lifelong calorie restriction. Mech Ageing Dev 130:297–307CrossRefPubMedPubMedCentralGoogle Scholar
- 40.Matsuyama S, Llopis J, Deveraux QL, Tsien RY, Reed JC (2000) Changes in intramitochondrial and cytosolic pH: early events that modulate caspase activation during apoptosis. Nat Cell Biol 6:318–325Google Scholar