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Apoptosis

, Volume 19, Issue 5, pp 789–800 | Cite as

17β-Estradiol protects against apoptosis induced by levofloxacin in rat nucleus pulposus cells by upregulating integrin α2β1

  • Si-Dong Yang
  • Lei Ma
  • Ti-Xin Gu
  • Wen-Yuan Ding
  • Feng Zhang
  • Yong Shen
  • Ying-Ze Zhang
  • Da-Long Yang
  • Di Zhang
  • Ya-Peng Sun
  • Yan-Li Song
Original Paper

Abstract

Levofloxacin has been reported to have cytotoxicity to chondrocytes in vitro. And 17β-estradiol has been widely studied for its protective effects against cell apoptosis. Based on apoptotic cell model induced by levofloxacin, the purpose of this study was to explore the mechanism by which 17β-estradiol protects rat nucleus pulposus cells from apoptosis. Inverted phase-contrast microscopy, flow cytometry, and caspase-3 activity assay were used to find that levofloxacin induced marked apoptosis, which was abolished by 17β-estradiol. Interestingly, estrogen receptor antagonist, ICI182780, and functional blocking antibody to α2β1 integrin, both prohibited the effect of 17β-estradiol. Simultaneously, levofloxacin decreased cellular binding ability to type II collagen, which was also reversed by 17β-estradiol. Furthermore, western blot and real-time quantitative PCR were used to find that integrin α2β1 was responsible for estrogen-dependent anti-apoptosis, which was time–response and dose–response effect. 17β-estradiol was proved for the first time to protect rat nucleus pulposus cells against levofloxacin-induced apoptosis by upregulating integrin α2β1 signal pathway.

Keywords

17β-Estradiol Levofloxacin Nucleus pulposus Integrin α2β1 Apoptosis 

Notes

Acknowledgments

None.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Si-Dong Yang
    • 1
    • 4
  • Lei Ma
    • 1
    • 4
  • Ti-Xin Gu
    • 2
  • Wen-Yuan Ding
    • 1
    • 4
  • Feng Zhang
    • 3
  • Yong Shen
    • 1
    • 4
  • Ying-Ze Zhang
    • 1
    • 4
  • Da-Long Yang
    • 1
    • 4
  • Di Zhang
    • 1
    • 4
  • Ya-Peng Sun
    • 1
    • 4
  • Yan-Li Song
    • 1
  1. 1.Department of Spine SurgeryThe Third Hospital of Hebei Medical UniversityShijiazhuangChina
  2. 2.Department of VIP WardThe First Hospital of Hebei Medical UniversityShijiazhuangChina
  3. 3.Department of Rehabilitation MedicineThe Third Hospital of Hebei Medical UniversityShijiazhuangChina
  4. 4.Hebei Provincial Key Laboratory of Orthopedic BiomechanicsShijiazhuangChina

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