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Cytoskeleton keratin regulation of FasR signaling through modulation of actin/ezrin interplay at lipid rafts in hepatocytes

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Abstract

FasR stimulation by Fas ligand leads to rapid formation of FasR microaggregates, which become signaling protein oligomerization transduction structures (SPOTS), through interactions with actin and ezrin, a structural step that triggers death-inducing signaling complex formation, in association with procaspase-8 activation. In some cells, designated as type I, caspase 8 directly activates effector caspases, whereas in others, known as type II, the caspase-mediated death signaling is amplified through mitochondria. Keratins are the intermediate filament (IF) proteins of epithelial cells, expressed as pairs in a lineage/differentiation manner. Hepatocyte IFs are made solely of keratins 8/18 (K8/K18), the hallmark of all simple epithelia. We have shown recently that in comparison to type II wild-type (WT) mouse hepatocytes, the absence of K8/K18 IFs in K8-null hepatocytes leads to more efficient FasR-mediated apoptosis, in link with a type II/type I-like switch in FasR-death signaling. Here, we demonstrate that the apoptotic process occurring in type I-like K8-null hepatocytes is associated with accelerated SPOTS elaboration at surface membrane, along with manifestation of FasR cap formation and internalization. In addition, the lipid raft organization is altered in K8-null hepatocytes. While lipid raft inhibition impairs SPOTS formation in both WT and K8-null hepatocytes, the absence of K8/K18 IFs in the latter sensitizes SPOTS to actin de-polymerization, and perturbs ezrin compartmentalization. Overall, the results indicate that the K8/K18 IF loss in hepatocytes alters the initial FasR activation steps through perturbation of ezrin/actin interplay and lipid raft organization, which leads to a type II/type I switch in FasR-death signaling.

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Acknowledgments

We thank H. Baribault for the original WT and K8-null mouse colonies, R. Kemler for the TROMA-1 hybridoma, J. Lippincott-Schwartz for the PAGFP vector and A. Helenius for the caveolin-1-mRFP construct. This work was supported by the Canadian Institutes of Health Research (MOP 15529 to NM; MOP 49450 to JNL).

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  1. Centre de Recherche en Cancérologie de l’Université Laval, and Centre de Recherche du Centre Hospitalier de Québec (CRCHUQ)/HDQ, 9 rue McMahon, Quebec, G1R 2J6, Canada

    Stéphane Gilbert, Anne Loranger, Josée N. Lavoie & Normand Marceau

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  1. Stéphane Gilbert
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  2. Anne Loranger
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  4. Normand Marceau
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Correspondence to Normand Marceau.

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10495_2012_733_MOESM1_ESM.tif

Online Resource 1: FasR capping is increased in absence of K8/K18 IFs. a Confocal imaging of FasR after the addition of FasL (100 ng/ml) for 60 min, showing more pronounced cap structure (white arrow) formation in K8-null than WT hepatocytes. b Quantification of the percentage of cells with caps at their surface as function of time following a stimulation with FasL (100 ng/ml) (TIFF 29865 kb)

10495_2012_733_MOESM2_ESM.tif

Online Resource 2: FasR internalization is observed only in cell without K8/K18 IFs. Confocal images of Jo2-PE at cell middle plan of WT and K8-null hepatocytes in presence of Jo2-PE (0.5 μg/ml) + PA (0.1 μg/ml), showing FasR internalization (red arrow in zoom panel) only in K8-null hepatocytes, starting at 30 min after being put back at 37 °C (TIFF 6852 kb)

10495_2012_733_MOESM3_ESM.tif

Online Resource 3: Faster FasR SPOTS formation in K8-null hepatocytes monitored by real-time confocal microscopy and alignment with caveolin-1. Real-time confocal imaging at the surface plan of WT (a) and K8-null (b) hepatocytes transfected with FasR-EGFP (green) and caveolin-1-mRFP (red), and stimulated with Jo2 (0.5 μg/ml) + PA (0.1 μg/ml), showing a faster FasR SPOTS formation (yellow circle) in K8-null compare to WT hepatocytes, and an alignment of caveolin-1 with FasR SPOTS. Images were taken each 30 s for a 30 min period (TIFF 11532 kb)

10495_2012_733_MOESM4_ESM.tif

Online Resource 4: Radixin co-distibrutes with actin and flotillin-2 at bile canaliculi but not with FasR. a Confocal imaging of radixin (green), actin (red) and nucleus (blue) in WT and K8-null hepatocytes, showing a co-distribution of actin and radixin at bile canaliculi of both cell types. b Confocal imaging of radixin (red), flotillin-2 (green) and nucleus (blue) in WT and K8-null hepatocytes, showing a co-distribution of flotillin-2 and radixin at bile canaliculi of both cell types; FasR stimulation led to the appearance of flotillin-2 at the surface membrane in K8-null hepatocytes, in co-localization with radixin. c Confocal imaging of radixin (green), FasR (red) and nucleus (blue) in WT and K8-null hepatocytes, revealing an absence of FasR and radixin co-localization (TIFF 21699 kb)

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Gilbert, S., Loranger, A., Lavoie, J.N. et al. Cytoskeleton keratin regulation of FasR signaling through modulation of actin/ezrin interplay at lipid rafts in hepatocytes. Apoptosis 17, 880–894 (2012). https://doi.org/10.1007/s10495-012-0733-2

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