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Vesicular stomatitis virus matrix protein gene enhances the antitumor effects of radiation via induction of apoptosis

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Abstract

Vesicular stomatitis virus (VSV) matrix (M) protein can directly induce apoptosis by inhibiting host gene expression when it is expressed in the absence of other viral components. Previously, we found that the M protein gene complexed to DOTAP-cholesterol liposome (Lip-MP) can suppress malignant tumor growth in vitro and in vivo; however, little is known regarding the biological effect of Lip-MP combined with radiation. The present study was designed to determine whether Lip-MP could enhance the antitumor activity of radiation. LLC cells treated with a combination of Lip-MP and radiation displayed apparently increased apoptosis compared with those treated with Lip-MP or radiation alone. Mice bearing LLC or Meth A tumors were treated with intratumoral or intravenous injections of Lip-MP and radiation. The combined treatment significantly reduced mean tumor volumes compared with either treatment alone in both tumor models and prolonged the survival time in Meth A tumor models and the intravenous injection group of LLC tumor models. Moreover, the antitumor effects of Lip-MP combined with radiation were greater than their additive effects when compared with the expected effects of the combined treatment in vivo. This study suggests that Lip-MP enhanced the antitumor activity of radiation by increasing the induction of apoptosis.

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Acknowledgements

This work was supported by National Key Basic Research Programme of China 2004CD518800, Project of National Natural Sciences Foundation of China, National 863 projects.

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Authors and Affiliations

  1. National Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan, 610041, People’s Republic of China

    Xiao-Bo Du, You Lu, Yan-Jun Wen, Ju-Mei Zhao, Peng Diao, Zhi-Ping Yuan, Bin Yao, Ling-Yu Fan, Guo-Qing Wang, Li Liu, Zhen-Yu Ding, Yong-Sheng Wang, Rui Wang, Yun-Qiu Mao, Bin Kan, Hong-Bin Wu, Hong-Xia Li, Han-Suo Yang, Hong-Bo Wu, Yu-Quan Wei & Xia Zhao

  2. Department of Oncology, MianYang Central Hospital, Mianyang, Sichuan, People’s Republic of China

    Xiao-Bo Du & Bin Yao

  3. Department of Radiation Oncology, Sichuan Cancer Hospital, Chengdu, Sichuan, People’s Republic of China

    Jin-Yi Lang, Peng Diao, Li Liu & Tao Li

  4. National Key Laboratory of Biotherapy and Cancer Center, School of Life Sciences, Sichuan University, Chengdu, Sichuan, People’s Republic of China

    Jian-Rong XU

  5. Department of Pharmacology, Yan’an Medical College, Suide, Shanxi, People’s Republic of China

    Ju-Mei Zhao

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  1. Xiao-Bo Du
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Correspondence to Xia Zhao.

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X-B. Du, J-Y. Lang and J-R XU––contributed equally to this work.

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Du, XB., Lang, JY., XU, JR. et al. Vesicular stomatitis virus matrix protein gene enhances the antitumor effects of radiation via induction of apoptosis. Apoptosis 13, 1205–1214 (2008). https://doi.org/10.1007/s10495-008-0253-2

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