Dexamethasone inhibits apoptosis in C6 glioma cells through increased expression of Bcl-XL

Abstract

The glucocorticoid dexamethasone (Dex) has been reported to modulate a number of signaling pathways and physiological processes, including apoptosis. This study was carried out to investigate the cytoprotective mechanism of Dex in C6 glioma cells. Pre-treatment of cells with Dex inhibited apoptosis induced by staurosporine, etoposide and thapsigargin. Apoptosis inhibition correlated with blockade of mitochondrial cytochrome c release, abolition of caspase-3 activity along with inhibition of caspase-9 and PARP cleavage. Dex-mediated cytoprotection coincided with the induction of the anti-apoptotic protein, Bcl-XL. The specific glucocorticoid receptor antagonist, RU486, reversed the anti-apoptotic effect of Dex and prevented Bcl-XL induction. Here, we show for the first time that knockdown of Bcl-XL expression with siRNA reversed the protective effects of the glucocorticoid in glioma cells. We conclude that Dex-mediated inhibition of apoptosis in C6 glioma cells is through induction of Bcl-XL.

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Abbreviations

Dex:

Dexamethasone

GAPDH:

Glyceraldehyde 3-phosphate dehydrogenase

GR:

Glucocorticoid receptor

GRE:

Glucocorticoid response element

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Correspondence to Afshin Samali.

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Ní Chonghaile, T., Concannon, C.G., Szegezdi, E. et al. Dexamethasone inhibits apoptosis in C6 glioma cells through increased expression of Bcl-XL. Apoptosis 11, 1247–1255 (2006). https://doi.org/10.1007/s10495-006-7233-1

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Keywords

  • Apoptosis
  • Bcl-XL
  • Dexamethasone
  • Glioma
  • siRNA