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Involvement of DNA replication in ultraviolet-induced apoptosis of mammalian cells

Apoptosis volume 11, Article number: 1139 (2006) Cite this article

Abstract

Exposure of cells to ultraviolet (UV) light damages the genome and the persistence of DNA lesions triggers apoptosis in mammalian cells. RNA transcription blockage by DNA damage is believed to be implicated in signaling for UV-induced apoptosis, but the role played by DNA replication in this process is still unclear. To address this point, we have employed the DNA polymerase inhibitor aphidicolin in UV-irradiated wild-type and XPB-mutated Chinese hamster ovary cells. The data obtained with synchronized cells indicate that induction of apoptosis by UV light is independent of the cell cycle phase. Nevertheless, cells treated with aphidicolin after UV exposure showed a significant prevention of apoptosis induction when compared to proliferating cells. These results were observed in both DNA-repair proficient and deficient cells, indicating that the prevention of apoptosis by aphidicolin is independent of the cells’ ability to repair the photolesions caused by UV. Taken together, these data suggest that replication of damaged DNA also leads to critical events signaling for UV-induced cell death.

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Author information

Author notes

  1. Vanessa Chiganças

    Present address: Laboratory of Genetic Instability and Cancer, Gustave Roussy Institute, France

  2. Gabriela Brumatti

    Present address: Department of Genetics, Trinity College, Dublin, Ireland

Affiliations

  1. Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, 05508-900, SP, Brazil

    Luis Francisco Zirnberger Batista, Vanessa Chiganças & Carlos Frederico Martins Menck

  2. Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, 05508-900, SP, Brazil

    Gabriela Brumatti & Gustavo Pessini Amarante-Mendes

  3. Immunology Investigation Institute—Millennium Institutes, Brazil

    Gustavo Pessini Amarante-Mendes

  4. Depto. de Microbiologia, ICB, USP, Av. Prof. Lineu Prestes, 1374. Ed. Biomédicas 2, São Paulo, 05508-900, SP, Brazil

    Carlos Frederico Martins Menck

Authors
  1. Luis Francisco Zirnberger Batista
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  2. Vanessa Chiganças
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  3. Gabriela Brumatti
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  4. Gustavo Pessini Amarante-Mendes
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  5. Carlos Frederico Martins Menck
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Corresponding author

Correspondence to Carlos Frederico Martins Menck.

Additional information

This work was supported by FAPESP (São Paulo, Brazil), CNPq (Brasília, Brazil), CAPES (Brasilia, Brazil) and COFECUB (Aix en Provence, France). CFMM is a Fellow from the John Simon Guggenheim Memorial Foundation (New York, USA).

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Batista, L.F.Z., Chiganças, V., Brumatti, G. et al. Involvement of DNA replication in ultraviolet-induced apoptosis of mammalian cells. Apoptosis 11, 1139 (2006). https://doi.org/10.1007/s10495-006-7109-4

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  • DOI: https://doi.org/10.1007/s10495-006-7109-4

Keywords

  • DNA replication
  • Apoptosis
  • Ultraviolet
  • Aphidicolin