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The anti-Fas antibody M-20 cross-reacts with intracellular proteins: implications for false-positive detection of Fas

Apoptosis volume 12, pages 459–462 (2007)Cite this article

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Abbreviations

TNFR:

tumor necrosis factor receptor

lpr:

lymphoproliferative

PVDF:

polyvinylidene fluoride

HRP:

horseradish peroxidase

DAB:

diamino benzidine

wt:

wild-type

KO:

knockout

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Affiliations

  1. Department of Physiology, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, Canada, H3G 1Y6

    Caroline Lambert & Julie Desbarats

Authors
  1. Caroline Lambert
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  2. Julie Desbarats
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Corresponding author

Correspondence to Julie Desbarats.

Additional information

This work was supported by Canadian Institutes of Health Research (CIHR) salary support grants to CL and JD and by CIHR grant 53337 (JD).

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Lambert, C., Desbarats, J. The anti-Fas antibody M-20 cross-reacts with intracellular proteins: implications for false-positive detection of Fas. Apoptosis 12, 459–462 (2007). https://doi.org/10.1007/s10495-006-0575-x

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  • DOI: https://doi.org/10.1007/s10495-006-0575-x

Keywords

  • Fas (CD95)
  • Antibody
  • Flow cytometry
  • Immunoblot
  • Immunohistochemistry
  • Cross-reactivity