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Modulation of Fas-induced apoptosis by p75 neurotrophin receptor in a human neuroblastoma cell line

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Abstract

Fas and p75 neurotrophin receptors (p75NTR) are death receptors that alone induce apoptosis of SH-SY5Y neuroblastoma cell line respectively by Fas ligand or brain-derived neurotrophic factor (BDNF, a p75NTR ligand). We report on the modulation of Fas-mediated apoptosis by concomitant p75NTR activation. The exposure to both ligands suppressed the apoptotic effect. A co-localisation of Fas and p75NTR receptors was evidenced by co-capping and immunoprecipitation assays. Moreover, a caspase-8 inhibitor suppressed the protective effect of the concomitant BDNF and Fas ligand stimulation, suggesting that p75NTR and Fas receptors could share common signalling pathways.

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Authors and Affiliations

  1. Laboratory of Immunology, EA 3842, University Hospital, 87042, Limoges, France

    S. Giraud, C. Lautrette, B. Bessette, C. Decourt, M. Mathonnet & M.-O. Jauberteau

  2. Laboratory of Immunology, University Hospital, 2 avenue Martin Luther King, 87042, Limoges, France

    M.-O. Jauberteau

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  1. S. Giraud
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  2. C. Lautrette
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  3. B. Bessette
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  4. C. Decourt
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Correspondence to M.-O. Jauberteau.

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Giraud, S., Lautrette, C., Bessette, B. et al. Modulation of Fas-induced apoptosis by p75 neurotrophin receptor in a human neuroblastoma cell line. Apoptosis 10, 1271–1283 (2005). https://doi.org/10.1007/s10495-005-2649-6

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