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Recruitment of beta-2-glycoprotein 1 to cell surfaces in extrinsic and intrinsic apoptosis

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Abstract

Apoptotic cells and phagocytes have developed a diverse array of distinct ligand-receptor systems that drive the recognition and uptake of dying cells. Phagocytes recognize apoptotic cells either directly, by binding to specific ligands at their cell surface, or indirectly, by binding to bridging proteins that bind these ligands. Previous observations showed that the plasma bridging protein β 2GP1, binds PS containing vesicles, and enhances their binding and engulfment by phagocytes in vitro. In this study we show that apoptotic cells injected intravenously and intraperitonealy into syngeneic mice recruited the PS binding protein, β 2GP1. Examination of peritoneal exudates and spleen thin sections showed that only the injected apoptotic cells picked up endogenous β 2GP1. Recovery of cells from the peritoneum showed that apoptotic cells bearing β 2GP1 were clustered around host peritoneal phagocytes. In addition, tissue sections from mice injected with Fas antibody showed colocalization of β 2GP1 with TUNEL-positive apoptotic cells. These results provide evidence that endogenous β 2GP1 binds apoptotic cells in vivo, suggesting that the protein plays an important physiologic role in the recognition of dying cells.

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Abbreviations

ActD:

actinomycin D

β 2GP1:

beta-2-glycopro- tein 1

PC:

phosphatidylcholine

PS:

phosphatidylserine

ROS:

reactive oxygen species

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Authors and Affiliations

  1. Department of Cancer Biology, The University of Texas, M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, Texas, 77030, USA

    K. Balasubramanian, S. N. Maiti & A. J. Schroit

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  1. K. Balasubramanian
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  2. S. N. Maiti
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  3. A. J. Schroit
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Correspondence to A. J. Schroit.

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Balasubramanian, K., Maiti, S.N. & Schroit, A.J. Recruitment of beta-2-glycoprotein 1 to cell surfaces in extrinsic and intrinsic apoptosis. Apoptosis 10, 439–446 (2005). https://doi.org/10.1007/s10495-005-0817-3

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  • DOI: https://doi.org/10.1007/s10495-005-0817-3

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