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The nuclear receptor gene E75 plays a key role in regulating the molting process of the spider mite, Tetranychus urticae

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Abstract

The nuclear receptor gene Ecdysone-induced protein 75 (E75), as the component of ecdysone response genes in the ecdysone signaling pathway, has important regulatory function for insect molting. However, the regulatory function of E75 during the molting process of spider mites is not yet clear. In this study, the expression pattern of E75 in the molting process of the spider mite Tetranychus urticae was analyzed. The results showed that there was a peak at 8 h post-molting, followed by a decline 8 h after entering each respective quiescent stage across various developmental stages. During the deutonymph stage, the expression dynamics of E75, observed at 4-h intervals, indicated that the transcript levels of TuE75 peaked at 24 h, coinciding with the onset of molting in the mites. To investigate the function of TuE75 during the molting process, silencing TuE75 through dsRNA injection into deutonymph mites at the age of 8 h yielded a notable outcome: 78% of the deutonymph mites were unable to progress to the adult stage. Among these phenotypic mites, 37% were incapable of transitioning into the quiescent state and eventually succumbed after a certain period. An additional 41% of the mites successfully entered the quiescent state but encountered difficulties in shedding the old epidermis, leading to eventual mortality. In summary, these results suggested that TuE75 plays a key role in the molting process of T. urticae.

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Funding

This research was supported by the Guizhou Provincial Science and Technology Foundation (QianKeHeJiChu ZK [2022]-YiBan065), the Special Natural Science Foundation of Guizhou University (Gui Da Te Gang He Zi [2022]13) and National Natrual Science Foundation of China (3175002).

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Authors and Affiliations

  1. Institute of Entomology, Guizhou University, Guiyang, 550025, China

    Zhuo Li, Liang Wang, Tianci Yi, Dongdong Liu, Gang Li & Dao-Chao Jin

  2. Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guiyang, 550025, China

    Zhuo Li, Liang Wang, Tianci Yi, Dongdong Liu, Gang Li & Dao-Chao Jin

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  1. Zhuo Li
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  2. Liang Wang
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  4. Dongdong Liu
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  5. Gang Li
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Contributions

Z. L., G. L., and D.C. J. conceived the study and participated in its design. L.W. performed the population of spider mites. Z.L. performed all of experiments. Z.L., T.C. Y., and G. L. analyzed the data. Z.L., D.C. J., and G. L. prepared the paper. All authors have read and agreed to the published version of manuscript.

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Correspondence to Gang Li or Dao-Chao Jin.

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Li, Z., Wang, L., Yi, T. et al. The nuclear receptor gene E75 plays a key role in regulating the molting process of the spider mite, Tetranychus urticae. Exp Appl Acarol 92, 1–11 (2024). https://doi.org/10.1007/s10493-023-00868-2

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