Abstract
Eriophyoids affect crops around the globe directly or indirectly as virus vectors. Eriophyoid systematics initiated over a century ago, yet more than 90% of their fauna remain undescribed. Morphological identification is challenging because of a limited number of traits, cryptic speciation and complex life cycle reported for many species in the group. Nucleic acids extraction for mite identification is challenging due to their microscopic size with researchers using pooled samples leading to polymorphisms and inconclusive results. Identification of mite virus vectors is a tiresome task that could be simplified with a protocol that allows for the detection of viruses in the individual specimen. This communication describes an innovative, highly efficient extraction and detection pipeline. Direct Reverse Transcriptase – Polymerase Chain Reaction (Drt-PCR) assays were implemented in the molecular identification of eriophyoids and detection of viruses present in their bodies. The reverse transcription step allows for amplification from a single mite or egg, as in addition to the genomic DNA, it incorporates the abundant transcripts of targeted genes, whereas it also allows for the amplification of viruses. This communication provides an efficient, sensitive and cost-effective alternative that can be implemented in pest identification and detection as well as biological and ecological studies.
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References
Amrine JW Jr (2002) Multiflora rose. In: Driesche FV, Blossey B, Hoodle M, Lyon S, Reardon R (eds) Biological control of invasive plants in the eastern United States. United States Department of Agriculture Forest Service. Forest Health Technology Enterprise Team, Morgantown, pp 265–292
Bouneb M, de Lillo E, Roversi PF, Simoni S (2014) Molecular detection assay of the bud mite Trisetacus juniperinus on Cupressus sempervirens in nurseries of central Italy. Exp Appl Acarol 62:161–170
Carew ME, Goodisman MA, Hoffmann AA (2004) Species status and population genetic structure of grapevine eriophyoid mites. Entomol Exp Appl 111:87–96
Carew M, Schiffer M, Umina P, Weeks A, Hoffmann A (2009) Molecular markers indicate that the wheat curl mite, Aceria tosichella Keifer, may represent a species complex in Australia. Bull Entomol Res 99:479–486. https://doi.org/10.1017/S0007485308006512
Casquet J, Thebaud C, Gillespie RG (2012) Chelex without boiling, a rapid and easy technique to obtain stable amplifiable DNA from small amounts of ethanol-stored spiders. Mol Ecol Resour 12:136–141. https://doi.org/10.1111/j.1755-0998.2011.03073.x
Castagnoli M, Lewandowski M, Łabanowski GS, Simoni S, Soika GM (2009) An insight into some relevant aspects concerning eriophyoid mites inhabiting forests, ornamental trees and shrubs. In: Ueckermann EA (ed) Eriophyoid mites: progress and prognoses. Springer, Dordrecht, pp 169–189
Chagas CM, Rossetti V, Colariccio A, Lovisolo O, Kitajima EW, Childers CC (2001) Brevipalpus mites (Acari: Tenuipalpoidae) as vectors of plant viruses. In: Halliday DE, Walter DE, Proctor H, Norton RA, Collof M (eds) Acarology: proceedings of the 10th international congress. CSIRO Publishing, Melbourne, pp 369–375
Chetverikov PE, Beaulieu F, Cvrkovic T, Vidovic B, Petanovic RU (2012) Oziella sibirica (Acari: Eriophyoidea: Phytoptidae), a new eriophyoid mite species described using confocal microscopy, COI barcoding and 3D surface reconstruction. Zootaxa 3560:41–60
Dabert J, Ehrnsberger R, Dabert M (2008) Glaucalges tytonis sp. n. (Analgoidea, Xolalgidae) from the barn owl Tyto alba (Strigiformes, Tytonidae): compiling morphology with DNA barcode data for taxon descriptions in mites (Acari). Zootaxa. https://doi.org/10.5281/zenodo.181058
Dabert M, Witalinski W, Kazmierski A, Olszanowski Z, Dabert J (2010) Molecular phylogeny of acariform mites (Acari, Arachnida): strong conflict between phylogenetic signal and long-branch attraction artifacts. Mol Phylogenet Evol 56:222–241
De Lillo E, Skoracka A (2010) What’s ‘cool’ on eriophyoid mites? Exp Appl Acarol 51:3-30. https://doi.org/10.1007/s10493-009-9297-4
De Lillo E, Craemer C, Amrine JW Jr, Nuzzaci G (2010) Recommended procedures and techniques for morphological studies of Eriophyoidea (Acari: Prostigmata). Exp Appl Acarol 51:283–307
Di Bello PL, Ho T, Tzanetakis IE (2015) The evolution of emaraviruses is becoming more complex: seven segments identified in the causal agent of Rose rosette disease. Virus Res 2:241–244. https://doi.org/10.1016/j.virusres.2015.08.009
Di Bello PL, Thekke-Veetil T, Druciarek T, Tzanetakis IE (2018) Transmission attributes and resistance to rose rosette virus. Plant Pathol 67(2):499–504
Druciarek T, Lewandowski M (2016) A new species of eriophyoid mite (Acari: Eriophyoidea) on Rosa sp. from Israel. Zootaxa. https://doi.org/10.11646/zootaxa.4066.3.8
Druciarek T, Lewandowski M, Kozak M (2014) Demographic parameters of Phyllocoptes adalius (Acari: Eriophyoidea) and influence of insemination on female fecundity and longevity. Exp Appl Acarol 63:349–360. https://doi.org/10.1007/s10493-014-9782-2
Fenton B, Malloch G, Moxey E (1997) Analysis of eriophyid mite rDNA internal transcribed spacer sequences reveals variable simple sequence repeats. Insect Mol Biol. https://doi.org/10.1046/j.1365-2583.1997.00152.x
Hassan M, Di Bello PL, Keller KE, Martin RR, Sabanadzovic S, Tzanetakis IE (2017) A new, widespread emaravirus discovered in blackberry. Virus Res. https://doi.org/10.1016/j.virusres.2017.04.006
Kozak M, Lewandowski M (2010) Eriophyoid mites (Acari: Eriophyoidea) on coniferous trees: is the occurrence of one species associated with the other? Exp App Acarol 50:115–122. https://doi.org/10.1007/s10493-009-9291-x
Kozlov MV, Skoracka A, Zverev V, Lewandowski M, Zvereva EL (2016) Two birch species demonstrate opposite latitudinal patterns in infestation by gall-making mites in northern Europe. PLoS ONE. https://doi.org/10.1371/journal.pone.0166641
Kumar PL, Fenton B, Duncan GH, Jones AT, Sreenivasulu P, Reddy DVR (2001) Assessment of variation in Aceria cajani using analysis of rDNA ITS regions and scanning electron microscopy: implications for the variability observed in host plant resistance to pigeonpea sterility mosaic disease. Ann Appl Biol 139:61–73. https://doi.org/10.1111/j.1744-7348.2001.tb00131.x
Lewandowski M, Skoracka A, Szydło W, Kozak M, Druciarek T, Griffiths DA (2014) Genetic and morphological diversity of Trisetacus species (Eriophyoidea: Phytoptidae) associated with coniferous trees in Poland: phylogeny, barcoding, host and habitat specialization. Exp Appl Acarol 63:497–520. https://doi.org/10.1007/s10493-014-9805-z
Lindquist EE (1996) 1.1.1 External Anatomy and Notation of Structures. In: Lindquist EE, Sabelis MW, Bruin J (eds) World Crop Pests, vol. 6. Elsevier, Amsterdam, pp 3–31
Lindquist EE, Amrine JW Jr. (1996) 1.1.2 Systematics, diagnoses for major taxa, and keys to families and genera with species on plants of economic importance. In: Lindquist EE, Sabelis MW, Bruin J (eds) World Crop Pests, vol. 6. Elsevier, Amsterdam, pp 33–87
Mielke-Ehret N, Thoma J, Schlatermund N, Mühlbach HP (2010) Detection of European mountain ash ringspot-associated virus-specific RNA and protein P3 in the pear leaf blister mite Phytoptus pyri (Eriophyidae). Arch Virol 155(6):987–991
Mironov SV, Dabert J, Dabert M (2012) A new feather mite species of the genus Proctophyllodes Robin, 1877 (Astigmata: Proctophyllodidae) from the long-tailed Tit Aegithalos caudatus (Passeriformes: Aegithalidae) – morphological description with DNA barcode data. Zootaxa 3253:54–61
Monfreda R, Lekveishvili M, Petanovic R, Amrine JW Jr (2010) Collection and detection of eriophyoid mites. Exp Appl Acarol 51:273–282. https://doi.org/10.1007/978-90-481-9562-6_14
Navajas M, Navia D (2010) DNA-based methods for eriophyoid mite studies: review, critical aspects, prospects and challenges. Expl App Acarol 51:257–271. https://doi.org/10.1007/978-90-481-9562-6_13
Navajas M, Ochoa R (2013) Integrating ecology and genetics to address Acari invasions. Exp Appl Acarol 59:1–10. https://doi.org/10.1007/s10493-012-9636-8
Petanović R (2016) Towards an integrative approach to taxonomy of Eriophyoidea (Acari, Prostigmata)—an overview. Ecol Mont 7:580–599
Rodrigues JCV, Peña JE (2010) Raoiella indica—facing it. In: Moraes GJ de, Castilho RC, Flechtmann CHW (ed) Abstract book. XIII International congress of acarology, Recife, PE, Brazil, p 319
Schulz JT (1963) Tetranychus telarius (L.) new vector of virus Y. Plant Dis Rept 47:594–596
Skoracka A, Dabert M (2010) The cereal rust mite Abacarus hystrix (Acari: Eriophyoidea) is a complex of species: evidence from mitochondrial and nuclear DNA sequences. Bull Entomol Res 100:263–272. https://doi.org/10.1017/S0007485309990216
Skoracka A, Magalhaes S, Rector BG, Kuczyński L (2015) Cryptic speciation in the Acari: a function of species lifestyles or our ability to separate species? Exp Appl Acarol 67:165–182. https://doi.org/10.1007/s10493-015-9954-8
Sonnenberg R, Nolte AW, Tautz D (2007) An evaluation of LSU rDNA D1-D2 sequences for their use in species identification. Front Zool 4:6. https://doi.org/10.1186/1742-9994-4-6
Stenger D, Hein G, Tatineni S, French R (2016) Eriophyid mite vectors of plant viruses. In: Brown JK (ed) Vector-mediated transmission of plant pathogens. APS Press, St. Paul, pp 263–274
Szydło W, Hein G, Denizhan E, Skoracka A (2015) Exceptionally high levels of genetic diversity in wheat curl mite (Acari: Eriophyidae) populations from Turkey. J Econ Entomol 108:2030–2039. https://doi.org/10.1093/jee/tov180
Tzanetakis IE, Keller KE, Martin RR (2005) The use of reverse transcriptase for efficient first-and second-strand cDNA synthesis from single-and double-stranded RNA templates. J Virol Meth 124:73–77. https://doi.org/10.1016/j.jviromet.2004.11.006
Xue XF, Guo JF, Dong Y, Hong XY, Shao R (2016) Mitochondrial genome evolution and tRNA truncation in Acariformes mites: new evidence from eriophyoid mites. Sci Rep 6:18920. https://doi.org/10.1038/srep18920
Zhang Z, Kermekchiev MB, Barnes WM (2010) Direct DNA amplification from crude clinical samples using a PCR enhancer cocktail and novel mutants of Taq. J Mol Diagn 12:152–161. https://doi.org/10.2353/jmoldx.2010.090070
Acknowledgments
This work was funded by USDA/APHIS/PPQ/14-8130-0404-CA and Hatch project 1002361. We thank Ava Wait for useful comments on a draft of this paper.
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Druciarek, T., Lewandowski, M. & Tzanetakis, I. A new, sensitive and efficient method for taxonomic placement in the Eriophyoidea and virus detection in individual eriophyoids. Exp Appl Acarol 78, 247–261 (2019). https://doi.org/10.1007/s10493-019-00382-4
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DOI: https://doi.org/10.1007/s10493-019-00382-4