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Differential expression analysis for subolesin in Rhipicephalus microplus infected with Anaplasma marginale

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Abstract

Rhipicephalus microplus (formerly Boophilus microplus) ticks are potential vectors of several pathogens of livestock especially in tropical and subtropical regions where may have substantial effects on economic development. Among tick-borne pathogens, Anaplasma marginale is considered one of the most important in domestic and wild ruminants worldwide. Different molecular mechanisms have been employed by both ticks and these intracellular pathogens, in order to be able to adapt and survive. Subolesin, originally called 4D8, is an evolutionarily well-preserved protein among ixodid tick species. This new antigen was found to be protective against tick infestations when used as a vaccine, as it has an essential role in tick blood digestion, development and infection of host cells by A. marginale. Recent studies have demonstrated that infection of both tick and vertebrate host cells with this microorganism changed gene expression. Therefore, the main objective of this study was to investigate subolesin expression in uninfected and A. marginale-infected R. microplus salivary glands by real-time reverse transcriptase (RT)-PCR. To analyze the differential expression of the recombinant protein subolesin, the gene was previously expressed from ticks infected with A. marginale. Results from this study revealed that, the expression of subolesin was significantly higher in salivary glands of infected R. microplus in comparison to uninfected ones.

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Acknowledgments

This work was supported by the Project CB-2015-01-255205: “Caracterización proteómica de Rhipicephalus microplus, R. annulatus y Amblyomma cajenennse para el control de infestaciones en bovinos”. Authorization DICB/C1000/3284/2016 funded by SEP-CONACYT.

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Authors and Affiliations

  1. Facultad de Agronomía y Veterinaria, Universidad Autónoma de San Luis Potosí, Km. 14.5 Carretera San Luis Potosí-Matehuala, Ejido Palma de la Cruz, CP 78321, Soledad de Graciano Sánchez, San Luis Potosí, S.L.P., Mexico

    Verónica Carvajal-de la Fuente & Fernando A. Muñoz-Tenería

  2. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, Km 5, Carretera Victoria-Mante, CP 87000, Ciudad Victoria, Tamaulipas, Mexico

    Verónica Carvajal-de la Fuente, Octavio Merino-Charrez & Erick Tovar-Carman

  3. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Cd. de México, México

    Sergio D. Rodríguez-Camarillo & Rodolfo E. Lagunes-Quintanilla

  4. SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ciudad Real, Spain

    Marinela Contreras & José de la Fuente

  5. Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, 74078, USA

    José de la Fuente

Authors
  1. Verónica Carvajal-de la Fuente
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  2. Octavio Merino-Charrez
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  3. Erick Tovar-Carman
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  4. Sergio D. Rodríguez-Camarillo
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  5. Rodolfo E. Lagunes-Quintanilla
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  6. Fernando A. Muñoz-Tenería
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  7. Marinela Contreras
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  8. José de la Fuente
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All authors have seen and approved the manuscript and have contributed significantly to the work.

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Correspondence to Verónica Carvajal-de la Fuente or José de la Fuente.

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Carvajal-de la Fuente, V., Merino-Charrez, O., Tovar-Carman, E. et al. Differential expression analysis for subolesin in Rhipicephalus microplus infected with Anaplasma marginale. Exp Appl Acarol 76, 229–241 (2018). https://doi.org/10.1007/s10493-018-0302-7

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