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Endosymbiont CLS-HI plays a role in reproduction and development of Haemaphysalis longicornis

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Abstract

Coxiella-like endosymbiont (CLS-Hl) is a primary endosymbiont of Haemaphysalis longicornis. CLS-Hl infects tick special tissues and its prevalence is 100% in ovaries and Malpighian tubules. Tetracycline was injected into females, which then fed on rabbits also treated with tetracycline. The densities of CLS-Hl were measured by semi-quantitative PCR. CLS-Hl densities in ovaries and Malpighian tubes of H. longicornis had significant effects on engorged weight, feeding time, number of eggs, oviposition period, and hatching period. These findings suggested that CLS-Hl plays a role in the reproduction and development of H. longicornis.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (Nos. 31672365, 31272372), Key Basic Research Foundation of Hebei Province, China (No. 15966502D).

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Author notes

  1. Chun-Mian Zhang and Ning-Xin Li contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China

    Chun-Mian Zhang, Ning-Xin Li, Tian-Tian Zhang, Zhao-Xi Qiu, Yuan Li & Jing-Ze Liu

  2. Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA

    Li-Wu Li

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  1. Chun-Mian Zhang
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  2. Ning-Xin Li
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  5. Yuan Li
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Correspondence to Jing-Ze Liu.

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Zhang, CM., Li, NX., Zhang, TT. et al. Endosymbiont CLS-HI plays a role in reproduction and development of Haemaphysalis longicornis . Exp Appl Acarol 73, 429–438 (2017). https://doi.org/10.1007/s10493-017-0194-y

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