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Increased activities of peroxidase and polyphenol oxidase enhance cassava resistance to Tetranychus urticae

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Abstract

In order to study the function of peroxidase (POD) and polyphenol oxidase (PPO) in cassava resistance to spider mites, we tested the changes of transcription levels and activities of these two protective enzymes in both cassava and Tetranychus urticae (=T. cinnabarinus) during the interaction. The results showed that after damage of the mite-susceptible cassava cultivar BRA900 by T. urticae for 1 and 8 days, the transcription levels of MePOD and MePPO and the activities of POD and PPO showed no significant difference compared with those in undamaged leaves. However, the corresponding transcription levels and activities in 1- and 8-day-damaged leaves of mite-resistant cassava cultivar C1115 increased to a significant level of approximately twofold. When T. urticae fed on BRA900 for 1 and 8 days, the transcription levels of TcPPO and TcPOD and the activities of PPO and POD showed no significant difference compared with those before feeding. However, the corresponding transcription levels and activities of these two protective enzymes in T. urticae feeding on C1115 significantly decreased by about half. This study preliminarily validates the function of POD and PPO in cassava resistance to T. urticae, and provides candidate gene resource for molecular breeding of spider mite-resistant cassava.

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Author notes

  1. Xiao Liang and Qing Chen have contributed equally to the work.

Authors and Affiliations

  1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, 571101, Hainan, China

    Xiao Liang, Qing Chen, Hui Lu, Chunling Wu, Fuping Lu & Jihong Tang

  2. Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, 571101, Hainan, China

    Xiao Liang, Qing Chen, Hui Lu, Chunling Wu, Fuping Lu & Jihong Tang

  3. Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture, Haikou, 571101, Hainan, China

    Xiao Liang, Qing Chen, Hui Lu, Chunling Wu, Fuping Lu & Jihong Tang

  4. Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests, Haikou, 571101, Hainan, China

    Xiao Liang, Qing Chen, Hui Lu, Chunling Wu, Fuping Lu & Jihong Tang

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  1. Xiao Liang
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  2. Qing Chen
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Correspondence to Qing Chen.

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Liang, X., Chen, Q., Lu, H. et al. Increased activities of peroxidase and polyphenol oxidase enhance cassava resistance to Tetranychus urticae . Exp Appl Acarol 71, 195–209 (2017). https://doi.org/10.1007/s10493-017-0125-y

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