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Experimental and Applied Acarology

, Volume 65, Issue 1, pp 55–71 | Cite as

Inter-population variation for Wolbachia induced reproductive incompatibility in the haplodiploid mite Tetranychus urticae

  • Eunho Suh
  • Cheolho Sim
  • Jung-Joon Park
  • Kijong ChoEmail author
Article

Abstract

Recent studies have revealed diverse patterns of cytoplasmic incompatibility (CI) induced by Wolbachia in the two spotted spider mite (Tetranychus urticae Koch). The mechanism of CI consists of two steps: modification (mod) of sperm of infected males and the rescue (resc) of these chromosomes by Wolbachia in the egg, which results in female embryonic mortality (FM), male development (MD) or no CI. Our study reports that Wolbachia infections were highly prevalent infecting all T. urticae populations from various crops in 14 commercial greenhouses in Korea, with two Wolbachia strains expressing distinctive phenotypic effects on hosts. Analyses for wsp gene sequences obtained from collected mite populations revealed all sequences were categorized into two groups (group W1 and W2) discriminated by three diagnostic nucleotides while all Wolbachia strains belonged to the subgroup Ori in Wolbachia supergroup B. Host plants of each mite population were also generally correlated this grouping. Various mating experiments with two mite populations from each group showed that CI patterns and host plants of the mite populations were completely matched with the grouping; no CI (modresc+) for group W1 and mixed pattern of FM and MD (mod+resc+) for group W2. No distinct changes in fecundity or sex ratio due to Wolbachia infections were observed in four mite populations regardless of Wolbachia grouping. Our study suggests a potential correlation between phenotypic effect of Wolbachia infection and its genetic diversity associated with host plants in Korean mite populations.

Keywords

Wolbachia Tetranychus urticae wsp Cytoplasmic incompatibility 

Notes

Acknowledgments

We thank the growers for their cooperation regarding mite sample collections. We thank Dr. Mun Il Ryoo and Dr. Ki Hyun Ryu for their helpful suggestions and technical support. This research is supported by Korea Ministry of Environment as “Climate Change Correspondence Program” and Korea University Special Grant to K. Cho.

Supplementary material

10493_2014_9846_MOESM1_ESM.docx (1.1 mb)
Supplementary material 1 (DOCX 1148 kb)

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Eunho Suh
    • 1
  • Cheolho Sim
    • 2
  • Jung-Joon Park
    • 3
  • Kijong Cho
    • 4
    Email author
  1. 1.Department of Biological SciencesVanderbilt UniversityNashvilleUSA
  2. 2.Department of BiologyBaylor UniversityWacoUSA
  3. 3.Department of Applied Biology, Institute of Agricultural and Life ScienceGyeongsang National UniversityJinjuSouth Korea
  4. 4.Division of Environmental Science and Ecological EngineeringKorea UniversitySeoulSouth Korea

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