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Experimental and Applied Acarology

, Volume 61, Issue 2, pp 213–219 | Cite as

Chlorophyll and its degradation products in the two-spotted spider mite, Tetranychus urticae: observations using epifluorescence and confocal laser scanning microscopy

  • Andrea Occhipinti
  • Massimo E. MaffeiEmail author
Article

Abstract

Chlorophyll and chlorophyll degradation products were observed in the two-spotted spider mite (Tetranychus urticae) using epifluorescence microscopy (EFM) and confocal laser scanning microscopy (CLSM). A clear red fluorescence (EFM) and a fluorescence induced by a laser wavelength of 650 nm (CLSM) were observed. In the lateral caeca, in the ventriculus and in the excretory organ, a bright light blue fluorescence was observed in close association with chlorophyll by using EFM. The same material can be localized with CLSM by using a laser with a wavelength of 488 nm. By comparison with synthetic guanine, this bright fluorescence is supposed to be guanine. The presence of guanine fluorescence in the mite pellets confirms this hypothesis. A possible mechanism for guanine formation is discussed.

Keywords

Tetranychus urticae Epifluorescence microscopy Confocal laser scanning microscopy Chlorophyll Guanine 

Supplementary material

Supplementary movie S1. This movie clip describes the pattern of guanine formation in spider mites. Guanine is formed in the lateral caeca (white circles) and is moved with peristaltic movements towards the central ventriculus. Here guanine accumulates and is merged in big guanine clusters that are stored in the excretory organ. Peristaltic movements inside the excretory organ aggregate more and more guanine pellets (yellow circles). Eventually 4-5 guanine clusters are formed and excreted. (MP4 5543 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of Life Sciences and Systems BiologyUniversity of Turin, Innovation CentreTurinItaly

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