Skip to main content

Advertisement

Springer Nature Link
Log in

Phenotype changes inherited by crossing pyrethroid susceptible and resistant genotypes from the cattle tick Riphicephalus (Boophilus) microplus

  • Published:
Experimental and Applied Acarology Aims and scope Submit manuscript

Abstract

Dialelic crosses and backcrosses of pyrethroid resistant (RR) and susceptible (SS) Rhipicephalus (Boophilus) microplus tick strains were carried out and the substitution (Phe-Ile) within the sodium channel gene was monitored in order to analyze the effects of the genotype on the pyrethroid resistance phenotype as measured by the larval packet test (LPT). Parental strains: susceptible (SS) and resistant (RR); dialelic crosses: RS (♂RR × ♀SS), and SR (♂SS × ♀RR); and backcrosses: RS × SS, RS × RR, SR × SS and SR × RR were infested on 280 kg calves. Resistance type (monogenic or polygenic) and effective dominance were determined based on the discriminant concentration (DC) for cipermethrine (0.5%), deltamethrine (0.09%) and flumethrine (0.01%). Allele specific PCR (AS-PCR) was used for genotyping, looking at a sodium channel mutation (Phe-Ile substitution). The mortality rates and allele frequency of susceptible and pyrethroid resistant reference strains were 0% mortality and 90% RR alleles for resistant strain, and 100% mortality and 0% RR alleles as measured by the larval packet test (LPT) and allele specific PCR (AS-PCR) respectively. Backcrossed strain SR × RR showed an effective dominance (DML) of 0.605 for cypermethrin, 0.639 for deltamethrin and 0.498 for flumethrin, while survival of backcrosses RS × SS, RS × RR and SR × SS showed a significant tendency to recesivity. Backcrossed strain SR × RR (69.4%) also showed a higher RR genotype frequency with regards to RS × SS (25.5%), RS × RR (36.7%) and SR × SS (32.0%), however, susceptible allele was inherited in general as an incomplete dominant trait. Monogenic inheritance hypothesis was tested and the results showed monogenic inheritance for cypermethrin and flumethrin (P < 0.05) but not for deltamethrin (P > 0.05). However, significant correlation was found between RR genotype and the survival rate for all three pyrethroids used (P < 0.05), suggesting that a single substitution on the sodium channel gene can be responsible for resistance to pyrethroids as a class, due to the high frequency for RR genotypes. Combination with different mutations or metabolic resistance mechanisms cannot be excluded.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  • Aguilar TG, Rodríguez VRI (2003) Effect of moxidectin against natural infestation of the cattle tick Boophilus microplus (Acarina: Ixodidae) in the Mexican tropics. Vet Parasit 11(2):211–216

    Article  Google Scholar 

  • Aguilar TG, Miller RJ, Hernández OR, Rodríguez VRI, Vásquez PC, García VZ, Olvera VF, Rosario CR (2008) Inheritance of pyrethroid resistance and a sodium channel gene mutation in the cattle tick Boophilus microplus. Parasit Res 103:633–639

    Article  Google Scholar 

  • Bourguet D, Genissel A, Raymond M (2000) Insecticide resistance and dominance levels. J Econ Entomol 93:1588–1595

    Article  PubMed  CAS  Google Scholar 

  • Brossard M, Wikel SK (2004) Tick immunobiology. Parasitology 129:161–176

    Article  Google Scholar 

  • Cen AJ, Rodríguez RI, Domínguez JA, Wagner G (1998) Studies on the effect on infectation by Babesia sp. on oviposition of Boophilus microplus engorged females naturally infected in the Mexican Tropics. Vet Parasit 78:253–257

    Article  Google Scholar 

  • Dong K (2007) Insect sodium channels and insecticide resistance. Invert Neurosci 7:17–30

    Article  PubMed  CAS  Google Scholar 

  • Field LM, Williamson MS (2001) Wider implications of new discoveries on the molecular basis of insecticide resistance. Research report 2000–2001. Institute of Arable Crops. pp 1–5

  • Guerrero FD, Pruett D (2003) Status and future prospects of molecular diagnosis of acaricide resistance in Boophilus microplus. Trends Entomol 3:97–103

    Google Scholar 

  • Guerrero FD, Davey RB, Miller RJ (2001) Use of an allele-specific polymerase chain reaction assay to genotype pyrethroid resistant strains of Boophilus microplus (Acari: Ixodidae). J Med Entomol 38:44–50

    Article  PubMed  CAS  Google Scholar 

  • Harris RL, George JE, Ahrens EH, Davey RB, Bazan HO (1988) Selection for resistance to coumaphos in a strain of southern cattle tick (Acari: Ixodidae). J Econ Entomol 81:545–548

    PubMed  CAS  Google Scholar 

  • He H, Chen AC, Davey RB, Ivie GW, George JE (1999) Identification of a point mutation in the para type sodium channel gene from a pyretroid-resistant cattle tick. Biochem Biophys Res Com 261:558–561

    Article  PubMed  CAS  Google Scholar 

  • Hernandez OR, Guerrero FD, George JE, Wagner GG (2002) Allele frecuency and gene expression of a putative carboxylesterase encoding gene in a pyrethroid resistant strain of the tick Boophilus microplus. Insect Biochem Mol Biol 32:1009–1016

    Article  PubMed  CAS  Google Scholar 

  • Jamroz RC, Guerrero FD, Pruett JH, Oehler DD, Miller RJ (2000) Molecular and biochemical survey of acaricide resistance mechanisms in larvae from Mexican strains of the southern cattle tick, Boophilus microplus. J Insect Physiol 46:685–695

    Article  PubMed  CAS  Google Scholar 

  • Kemp DH, Thulner F, Gale RK, Nari A, Sabatini GA (1998) Acaricide resistance in the cattle ticks Boophilus microplus and Boophilus decoloratus. Report to the Animal Health Services Food and Agriculture Organization of the United Nations. pp 1–32

  • Li YA, Davey BR, Miller JR, George J (2005) Mode of inheritance of amitraz resistance in a Brazilian strain of the southern cattle tick, Boophilus microplus (Acari: Ixodidae). Exp Appl Acarol 37:183–198

    Article  PubMed  CAS  Google Scholar 

  • Li YA, Davey BR, Miller JR, Guerrero FD, George J (2008) Genetics and mechanisms of a permethrin resistance in the Santa Luiza strain of Boophilus microplus (Acari: Ixodidae). J Med Entomol 45(3):427438

    Google Scholar 

  • Mebrahtu YB, Norem J, Taylor M (1997) Inheritance of larval resistance to permethrin in Aedes aegypti and association with sex ratio distortion and life history variation. Am J Trop Med Hyg 56:456–465

    PubMed  CAS  Google Scholar 

  • Miller RJ, Davey RB, George JE (1999) Characterization of pyrethroid resistance and susceptibility to coumaphos in Mexican Boophilus microplus (Acari: Ixodidae). J Med Entomol 36:533–538

    PubMed  CAS  Google Scholar 

  • Rodríguez VRI, Quiñones AF, Fragoso SH (2005) Epidemiología y control de la garrapata Boophilus en México. En: Enfermedades de importancia económica en producción animal. Edit. McGraw-Hill-UADY, México, pp 571–592

    Google Scholar 

  • Rodríguez VRI, Alonso DMA, Rodríguez AF, Fragoso SH, Santamaría VM, Rosario CR (2006) Prevalence and potential risk factors for organophosphate and pyrethroid resistance in Boophilus microplus ticks on cattle ranches from the state of Yucatan, Mexico. Vet Parasit 136:335–442

    Article  Google Scholar 

  • Rodríguez VRI, Arieta RJR, Pérez CLC, Rosado AJA, Ramírez CGT, Basto EG (2010, in press) Uso de lactonas macrocíclicas para el control de la garrapata Rhipicephalus (Boophilus) microplus en el ganado bovino. Archivos de Medicina Veterinaria

  • Rosario-Cruz R, Guerrero FD, Miller RJ, Rodríguez VRI, Domínguez GDI, Cornel AJ, Hernández OR, George JE (2005) Roles played by esterase activity and by a sodium channel mutation involved in pyrethroid resistance in populations of Boophilus microplus (Canestrini) (Acari: Ixodidae) collected from Yucatan, Mexico. J Med Entomol 42(6):1020–1025

    Article  PubMed  CAS  Google Scholar 

  • Rosario-Cruz R, Guerrero FD, Miller RJ, Rodríguez VRI, Tijerina M, Domínguez GDI, Hernández OR, Cornel AJ, McAbee RD, Alonso DMA (2009) Molecular survey of pyrethroid resistance mechanisms in Mexican field populations of Rhipicephalus (Boophilus) microplus. Parasit Res 105(4):1145–1153

    Article  Google Scholar 

  • SAS Software Systems for Windows Version Eight (1999)

  • Soderlund DM, Knipple DC (2003) The molecular biology of knockdown resistance to pyrethroid insecticide. Insect Biochem Mol Biol 33:563–577

    Article  PubMed  CAS  Google Scholar 

  • Sokal R, Rohlf R (1981) Biometry, 2nd edn. Freeman, San Francisco, pp 123–128

    Google Scholar 

  • SPSS. Ver. 13 Paquete estadístico para Windows (2004)

  • Stone BF, Haydock P (1962) A method for measuring the acaricide susceptibility of the cattle tick Boophilus microplus. Bull Entomol 53:563–578

    Article  CAS  Google Scholar 

  • Stone BF, Youlton NJ (1982) Inheritance of resistance to chlorpyrifos in the Mt Alford strain and to diazinon in the gracemera strain of the cattle tick (Boophilus microplus). Aust J Biol Sci 35:427–440

    PubMed  CAS  Google Scholar 

  • Tabashnik BE (1991) Determining the mode of inheritance of pesticide resistance with backcross experiments. J Econ Entomol 84(3):703–712

    PubMed  CAS  Google Scholar 

  • Tapia PG, García VZ, Montaldo H, George JE (2003) Inheritance of resistance to flumethrin in the Mexican Aldama strain of cattle tick Boophilus microplus (Acari: Ixodidae). Exp Appl Acarol 31:135–149

    Article  Google Scholar 

Download references

Acknowledgments

Special aknowledments to Edgar Castro Saines, Delia Inés Dominguez García and Mary Tijerina for their technical assistance and We acknowledge the Mexican National Council of Science and Technology and the Universidad Autonoma de Chiapas for the scholarship granted to Gabriela Aguilar Tipacamu for her pursued doctoral degree at the Veterinary Medicine College from the Universidad Nacional Autonoma de Mexico. This project was partially funded by the Mexican National Council of Science and Technology through the Complementary financial support program contract No. 90195 and FOMIX Guerrero contract No. 92367/2008-1 both granted to Dr. Rodrigo Rosario-Cruz.

Author information

Authors and Affiliations

  1. FMVZ-UNACH, Camino Ejido Emiliano Zapata Km. 8, Tuxtla Gutiérrez, Chiapas, Mexico

    G. Aguilar-Tipacamú

  2. Rodrigo Rosario Cruz, INIFAP, CENID-PAVET, Carr. Fed. Cuernavaca-Cuautla # 8534, Col. Progreso, C.P. 62550, Jiutepec, Morelos, Mexico

    R. Rosario-Cruz & Z. García-Vázquez

  3. Cattle Fever Ticks Research Laboratory USDA-ARS, 22675, North Moorefield Rd., Bldg. 6419, Edinburg, TX, 78541, USA

    Robert J. Miller

  4. Knipling-Bushland US Livestock Insect Research Laboratory, USDA-ARS, 2700 Fredericksburg Road, Kerrville, TX, 78028, USA

    Felix D. Guerrero

  5. FMVZ-UADY, Km. 15.5 carretera Mérida-Xmatkuil, C.P.97100, Mérida, Yucatán, Mexico

    R. I. Rodriguez-Vivas

Authors
  1. G. Aguilar-Tipacamú
    View author publications

    Search author on:PubMed Google Scholar

  2. R. Rosario-Cruz
    View author publications

    Search author on:PubMed Google Scholar

  3. Robert J. Miller
    View author publications

    Search author on:PubMed Google Scholar

  4. Felix D. Guerrero
    View author publications

    Search author on:PubMed Google Scholar

  5. R. I. Rodriguez-Vivas
    View author publications

    Search author on:PubMed Google Scholar

  6. Z. García-Vázquez
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to R. Rosario-Cruz.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aguilar-Tipacamú, G., Rosario-Cruz, R., Miller, R.J. et al. Phenotype changes inherited by crossing pyrethroid susceptible and resistant genotypes from the cattle tick Riphicephalus (Boophilus) microplus . Exp Appl Acarol 54, 301–311 (2011). https://doi.org/10.1007/s10493-011-9441-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10493-011-9441-9

Keywords