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Antigen-presenting cells in draining lymph nodes of goats repeatedly infested by the Cayenne tick Amblyomma cajennense nymphs

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Abstract

Resistance to tick feeding has been previously shown to be an acquired, immunologically mediated phenomenon in goats, associated with cutaneous basophilia to nymphs of Amblyomma cajennense, the Cayenne tick, after repeated infestations. On the other hand, it is well known that antigen-presenting cells (APCs) play an important role in the host immune reaction to tick infestations. The most able APCs for Th cells are the well defined dendritic cells, mononuclear phagocytes and B-lymphocytes. Immunohistochemical analysis of draining lymph nodes of goats repeatedly infested with nymphs of the ixodid tick A. cajennense to search for APCs was done. Pre-scapular lymph nodes draining the tick attachment sites were collected 15 days after both the first and third infestations. Tick infestations resulted in increased number of CD21+ B lymphocytes in lymph nodes after the tertiary infestation. However, the number of CD11b+ and CD11c+ cells were not altered after the successive infestations. Lower numbers of CD11c+ cells had infiltrated lymph nodes responsible for draining the tick infested skin. These findings suggest that acquired immunity of goats against nymphs of A. cajennense is possibly established by B lymphocytes during the first infestation and that APCs may play a key role in this mechanism.

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Acknowledgments

Authors wish to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico–CNPq and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–CAPES for G.H. Bechara, G.E.R. Monteiro and A.M. Franzin scholarships, and Mrs. Maria Inês Yamazaki de Campos and Mr. Ronaldo E. del Vecchio for their excellent technical assistance. Part of this work has been facilitated through the International Consortium on Ticks and Tick-borne Diseases (ICTTD-3) Coordination Action financed by the INCO program of the European Commission Project No.510561.

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Authors and Affiliations

  1. Faculty of Veterinary Medicine, University Eduardo Mondlane-UEM, Maputo, Mozambique

    Gaby Ermelindo Roberto Monteiro

  2. Department of Animal Pathology, Faculty of Agronomic and Veterinary Sciences, São Paulo State University-UNESP, Via de Acesso Professor Paulo D. Castellane, s/n, Jaboticabal, SP, 14884-900, Brazil

    Gervásio Henrique Bechara

  3. Faculty of Medicine of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, SP, Brazil

    Alessandra Maria Franzin & Isabel Kinney Ferreira de Miranda Santos

  4. National Centre for Genetic Resources and Biotechnology, Brazilian Enterprise for Agricultural Research, Brasília, DF, Brazil

    Isabel Kinney Ferreira de Miranda Santos

Authors
  1. Gaby Ermelindo Roberto Monteiro
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  2. Gervásio Henrique Bechara
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  3. Alessandra Maria Franzin
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  4. Isabel Kinney Ferreira de Miranda Santos
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Correspondence to Gervásio Henrique Bechara.

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Monteiro, G.E.R., Bechara, G.H., Franzin, A.M. et al. Antigen-presenting cells in draining lymph nodes of goats repeatedly infested by the Cayenne tick Amblyomma cajennense nymphs. Exp Appl Acarol 53, 63–69 (2011). https://doi.org/10.1007/s10493-010-9380-x

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  • DOI: https://doi.org/10.1007/s10493-010-9380-x

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