Abstract
A study on 12 entomopathogenic fungi for controlling broad mite (Polyphagotarsonemus latus (Banks)) in mulberry found that Metarhizium anisopliae CKM-048 was the most virulent strain in controlling both larvae and adult broad mites at the concentration of 2 × 108 conidia/ml. There was no ovicidal effect when tested with broad mite eggs. Median lethal concentrations (LC50) of M. anisopliae in killing larvae and adults were 8.7 × 106 and 1.3 × 107 conidia/ml, respectively. Median lethal times (LT50) of larvae and adults were 2.4 and 3.8 days, respectively, at the concentration of 2 × 108 conidia/ml. The fungus was found to produce protease and chitinase. Scanning electron microscope (SEM) studies were done to monitor the infection steps of the fungus on broad mites. A greenhouse test on mulberry trees revealed that M. anisopliae could reduce the broad mite population within 4 days after treatment. However, after 7 days, its efficacy was decreased significantly.
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Acknowledgements
We would like to thank the following people and institutions for their contributions: Dr. Tewin Kulpiyawat (Thai Department of Agriculture) for supporting the broad mite culture, Ms. Lawan Chatanon (TISTR) for fungal identification, and the Enzyme Technology and Waste Management Research Unit (Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, KAPI) for enzyme analyses.
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Maketon, M., Orosz-Coghlan, P. & Sinprasert, J. Evaluation of Metarhizium anisopliae (Deuteromycota: Hyphomycetes) for control of broad mite Polyphagotarsonemus latus (Acari: Tarsonemidae) in mulberry. Exp Appl Acarol 46, 157–167 (2008). https://doi.org/10.1007/s10493-008-9153-y
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DOI: https://doi.org/10.1007/s10493-008-9153-y