Effects of azadirachtin on the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae)

  • Elena Martínez-Villar
  • Francisco J. Sáenz-De-Cabezón
  • Fernando Moreno-Grijalba
  • Vicente Marco
  • Ignacio Pérez-Moreno


Effects of sublethal azadirachtin exposure to the biological performance of Tetranychus urticae Koch was studied under laboratory conditions. Bioassay was used to asses the effect of different concentrations of azadirachtin on longevity, fecundity, fertility, and offspring development. Azadirachtin (64 and 128 ppm) affected fecundity and mortality but had no effect on fertility and offspring development. A subsequent life-table study with 80 ppm of azadirachtin found that the compound caused a reduction of 50% in survival to adult stage. The peak of reproduction was reached at 5 days causing a decrease in the mean fecundity to almost eight times than of untreated females. The net reproductive rate (R 0), the intrinsic rate of increase (r m), and the finite rate of increase (λ) of treated females were lower. Treatment showed a negative value of r m, resulting in a declining population. These results suggest that azadirachtin could be incorporated in Integrated Pest Management (IPM) programmes of T. urticae.


Azadirachtin Demographic toxicology Intrinsic rate of increase Life-table parameters Spider mite Tetranychus urticae 


  1. Ahmadi, A. 1983Demographic toxicology as a method for studying the dicofol-twospotted spider mite (Acari: Tetranychidae) systemJ. Econ. Entomol.76239242Google Scholar
  2. Allan, J.D., Daniels, R.E. 1982Life table evaluation of chronic exposure of Eurytemora affinis (Copepoda) to KeponeMar. Biol.66179184CrossRefGoogle Scholar
  3. Amason, J.T., Philogene, B.J.R., Morand, P. 1989Insecticides of Plant OriginAmer. Chem. Soc. Symp. No 387. Am. Chem. SocWasington, DC213Google Scholar
  4. Ascher, K.R.S. 1993Nonconventional. insecticidal effects of pesticides available from the neem treeAzadirachta indica Arch. Ins. Biochem. Physiol.22433449CrossRefGoogle Scholar
  5. Boykin, L.S., Campbell, W.M. 1982Rate of population increase of the twospotted spider mite (Acari: Tetranychidae) on peanut leaves treated with pesticidesJ. Econ. Entomol.75966971Google Scholar
  6. Dimetry, N.Z., Amer, S.A.A., Reda, A.S. 1993Biological activity of two neem seed kernel extracts against the two-spotted spider mite Tetranychus urticae KochJ. Appl. Entomol.116308312Google Scholar
  7. Ibrahim, Y.B., Knowles, C.O. 1986Effects of formamidines on the population growth of Tetranychus urticae KochCrop Prot.5411416CrossRefGoogle Scholar
  8. Jeppson, L.R., Keifer, H.H., Baker, E.W. 1975Mites Injurious to Economic PlantsUniversity of California PressBerkeley, CA458Google Scholar
  9. Jones, V.P., Parrella, M.P. 1984The sublethal effects of selected insecticides on life-table parameters of Panonychus citri (Acari: Tetranychidae)Can. Entomol.11610331040CrossRefGoogle Scholar
  10. Makundi, R.H., Kashenge, S. 2002Comparative efficacy of neem Azadirachta indica extract formulations and the synthetic acaricide Amitraz (Mitac) against the two spotted spider miteTetranychus urticae (Acari: Tetranychidae), on tomatoes, Lycopersicum aesculentum Z. Pflanzenk. Pflanzen.1095763Google Scholar
  11. Marcic, D. 2003The effects of clofentezine on life-table parameters in two-spotted spider mite Tetranychus urticae Exp. Appl. Acarol.30249263CrossRefPubMedGoogle Scholar
  12. Meyer, J.S., Ingersoll, C.G., McDonald, L.L., Boyce, M.S. 1986Estimating uncertainty in population growth rates: Jackknife vs. Bootstrap techniquesEcology6711561166Google Scholar
  13. Mordue, A.J., Blackwell, A. 1993Azadirachtin: an updateJ. Insect Physiol.39903924CrossRefGoogle Scholar
  14. Petitt, F.L., Loader, A., Schon, M.K. 1994Reduction of nitrogen concentration in the hydroponic solution on population growth rate of the aphids (Homoptera: Aphididae) Aphis gossyppi on cucumber and Myzus persicae on pepperEnviron. Entomol.23930936Google Scholar
  15. Potter, C. 1952An improved laboratory apparatus for applying direct sprays and surface films, with data on the electrostatic charge on atomized sprays fluidsAnn. Appl. Biol.1129CrossRefGoogle Scholar
  16. Robertson, J.L., Preisler, H.K. 1992Pesticide Bioassays with ArthropodsCRC PressBoca Raton, Florida127Google Scholar
  17. Robertson, J.L., Worner, S.P. 1990Population toxicology: suggestion for laboratory bioassays to predict pesticide efficacyJ. Econ. Entomol.83812Google Scholar
  18. Sanguanpong, U., Schmutterer, H. 1992Laboratory trials on the effects of neem oil and neem-seed based extracts against the 2-spotted spirder-mite Tetranychus urticae Koch (Acari: Tetranychidae)Z. Pflanzenk. Pflanzen.99637646Google Scholar
  19. Schmutterer, H. 1990Properties and potential of natural pesticides from the neem treeAzadirachta indica Ann. Rev. Entomol.35271297CrossRefGoogle Scholar
  20. SPSS 1999. SPSS 10.0.6 copyright SPSS Inc. Google Scholar
  21. Stark, J.D., Banks, J.E. 2003Population-level effects of pesticides and other toxicants on arthropodsAnnu. Rev. Entomol.48505519CrossRefPubMedGoogle Scholar
  22. Stark, J.D., Wennergren, U. 1995Can population effects of pesticides be predicted from demographic toxicological studies?J. Econ. Entomol.8810891096Google Scholar
  23. Stark, J.D., Tanigoshi, L., Bounfour, M., Antonelli, A. 1997Reproductive potential: its influence on the susceptibility of a species to pesticidesEcotoxicol. Environ. Saf.37273279CrossRefPubMedGoogle Scholar
  24. Sundaram, K.M.S. 1996Azadirachtin biopesticide: a review of studies conducted on its analytical chemistry, environmental behaviour and biological effectsJ. Environ. Sci. HealthB31913948Google Scholar
  25. Sundaram, K.M.S., Sloane, L. 1995Effects of pure and formulated azadirachtin, a neem-based pesticide on the phytophagous spider miteTetranychus urticae KochJ. Environ. Sci. HealthB30801814Google Scholar
  26. Taberner, A., Castañera, P., Silvestre, E., Bopazo, J. 1993Estimation of the intrinsic rate of natural increase and its error by both algebraic and resampling approachesComput. Appl. Biosci.9535540PubMedGoogle Scholar
  27. Young-Joon, A., Min, K., Jai-Ki, Y., Sang-Ji, B. 1993Toxicity of flufenoxuron alone and in mixture with alphacypermethrin or fenbutatin oxide to Tetranychus urticae Panonychus u1mi (Acari: Tetranychidae)J. Econ. Entomol.8613341338Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Elena Martínez-Villar
    • 1
  • Francisco J. Sáenz-De-Cabezón
    • 1
  • Fernando Moreno-Grijalba
    • 1
  • Vicente Marco
    • 1
  • Ignacio Pérez-Moreno
    • 1
  1. 1.Departamento de Agricultura y AlimentaciónUniversidad de La RiojaLogroñoSpain

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