Abstract
In this research, two novel Fe(III)-reducing bacteria, SG10T and SG198T of genus Geothrix, were isolated from the rice field of Fujian Agriculture and Forestry University in Fuzhou, Fujian Province, China. Strains SG10T and SG198T were strictly anaerobic, rod-shaped and Gram-stain-negative. The two novel strains exhibited iron reduction ability, utilizing various single organic acid as the elector donor and Fe(III) as a terminal electron acceptor. Strains SG10T and SG198T showed the highest 16S rRNA sequences similarities to the type strains of Geothrix oryzisoli SG189T (99.0–99.5%) and Geothrix paludis SG195T (99.0–99.7%), respectively. The phylogenetic trees based on the 16S rRNA gene and genome 120 conserved core genes showed that strains SG10T and SG198T belong to the genus Geothrix. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the phylogenetic neighbors and the two isolated strains were 86.1–94.3% and 30.7–59.5%, respectively. The major fatty acids were iso-C15:0, anteiso-C15:0, C16:0 and iso-C13:0 3OH, and MK-8 was the main respiratory quinone. According to above results, the two strains were assigned to the genus Geothrix with the names Geothrix campi sp. nov. and Geothrix mesophila sp. nov. Type strains are SG10T (= GDMCC 1.3406 T = JCM 39331 T) and SG198T (= GDMCC 62910 T = KCTC 25635 T), respectively.
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Abbreviations
- ANI:
-
Average nucleotide identity
- dDDH:
-
Digital DNA–DNA hybridization
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Acknowledgements
We are grateful to Prof. A. Oren for his kindly help and advice on nomenclature.
Funding
This work was supported by the National Science Fund for Excellent Young Scholars of China (grant: 42222703), National Natural Science Foundation of China (grant: 42277257) and Natural Science Foundation of Fujian Province (grant: 2020J02015 and 2023J01202).
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SGZ and GHL designed research, project outline and revised the manuscript. XL revised the manuscript. SH drafted the manuscript. SH, SY and CJX performed deposition and polyphasic taxonomy. RT performed isolation.
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The GenBank accession numbers for the 16S rRNA gene and genome sequences of strains SG10T and SG198T were OP023989 and JANHMP000000000, OM855572 and JAKZLD000000000, respectively.
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Han, S., Yang, S., Tang, R. et al. Two novel Fe(III)-reducing bacteria, Geothrix campi sp. nov. and Geothrix mesophila sp. nov., isolated from paddy soils. Antonie van Leeuwenhoek 117, 68 (2024). https://doi.org/10.1007/s10482-024-01967-9
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DOI: https://doi.org/10.1007/s10482-024-01967-9