Abstract
Translation elongation factor P, expressed by the efp gene, is a conserved protein closely related to bacterial virulence and environmental stress regulation responses, however, little is known about the efp gene expression regulations. Here, the strain of Staphylococcus aureus subsp. aureus NCTC 8325 was taken as the research object and cultured under different conditions, including different culture temperatures, pH, and antibiotics, to study the expression of the efp gene in S. aureus by qRT-PCR, the results showed that the expression of the efp gene is upregulated under high temperature (40 °C), acidic (pH 5.4) or alkaline (pH 9.4) culture conditions, but upregulated early and downregulated later under the conditions of 0.5 MIC antibiotics (chloramphenicol at the final concentration of 2 μg/mL and vancomycin at the final concentration of 0.25 μg/mL), indicating that the efp promoter in S. aureus is inducible. The efp promoter sequence and structure in S. aureus were predicted by bioinformatics methods, and the predicted promoter was validated by constructing a promoter-probe vector and a series of promoter mutants, the results showed that the efp promoter sequence in S. aureus, named Pro, located in 1,548,179–1,548,250 of the S. aureus genome (NC_007795.1), and the sequence of − 10 element is CCTTATAGT, − 35 element is TTTACT. The results above could lay a foundation for screening transcription factors involved in the expression of the efp gene and then exploring the transcriptional regulation mechanism of EF-P in S. aureus.
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This research was supported financially by the National Natural Science Foundation of China (Grants 31960015) and the Natural Science Foundation of Jiangxi Province, China (Grants 20192BAB204001).
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Xingxing Zheng, Xiuhui Sun, Weiwei Xiang, Haiyan Ni, and Long Zou. Zhong-er Long, Xingxing Zheng, and Xiuhui Sun wrote the manuscript. All authors read and approved the final manuscript.
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Zheng, X., Sun, X., Xiang, W. et al. Expression of Staphylococcus aureus translation elongation factor P is regulated by a stress-inducible promotor. Antonie van Leeuwenhoek 117, 54 (2024). https://doi.org/10.1007/s10482-024-01954-0
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DOI: https://doi.org/10.1007/s10482-024-01954-0