Abstract
A strictly anaerobic, Gram-stain-negative, catalase-negative, cocci-shaped, and propionate-producing bacterial strain, named Ds1651T was isolated from the fecal sample collected from a South Korean infant. Through a comparison of 16S rRNA gene sequences, it was revealed that Ds1651T had the highest phylogenetic affinity with Veillonella nakazawae KCTC 25297 T (99.86%), followed by Veillonella infantium KCTC 25370 T (99.80%), and Veillonella dispar KCTC 25309 T (99.73%) in the family Veillonellaceae. Average nucleotide identity values between Ds1651T and three reference species were 95.48% for Veillonella nakazawae KCTC 25297 T, 94.46% for Veillonella infantium KCTC 25370 T, and 92.81% for Veillonella dispar KCTC 25309 T. The G + C content of Ds1651T was 38.58 mol%. Major fermentation end-products were acetic and propionic acids in Trypticase peptone glucose yeast extract broth with 1% (v/v) sodium lactate. The predominant cellular fatty acids that account for more than 10% were summed in Feature 8 (C17:1 ω8c and/or C17:2) and C13:0. Based on the findings from phylogenetic, genomic, phenotypic, and chemotaxonomic studies, we propose that the type strain Ds1651T (= KCTC 25477 T = GDMCC 1.3707 T) represents a novel bacterial species within the genus Veillonella, with the proposed name Veillonella faecalis sp. nov.
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Data availability
The authors confirm that the data supporting the findings of the current study are available in the article and supplementary material. The 16S rRNA gene sequence and draft genome were available from the National Center for Biotechnology Information.
Abbreviations
- TPGY:
-
Trypticase peptone glucose yeast extract liquid medium
- TPGYL:
-
Trypticase peptone glucose yeast extract liquid medium (TPGY) supplemented with 1% (w/v) sodium lactate
- TYHG:
-
Trypticase-yeast extract-hemin (TYH) liquid medium supplemented with 1% (w/v) glucose
- TYHL:
-
Trypticase-yeast extract-hemin (TYH) liquid medium supplemented with 1% (w/v) sodium lactate
- RCM:
-
Reinforced clostridial medium
- ANI:
-
Average nucleotide identity
- dDDH:
-
Digital DNA-DNA hybridization
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Funding
This work was supported by a Korea Innovation Foundation (INNPOLIS) grant funded by the Korean government (MSICT) through a science and technology project that opens the future of the region (2021-DD-UP-0380), and a grant from the KRIBB Research Initiative Program (KGM5232221).
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HY carried out the experiment, data analysis, and wrote the manuscript; HJ and SHK helped perform the experiment and data interpretation; C-SY contributed to isolation of strain Ds1651; S-HP and D-SP contributed to the conception and design of the study and revised the manuscript.
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Yang, H., Jo, H., Kim, S.H. et al. Veillonella faecalis sp. nov., a propionic acid-producing bacterium isolated from the faeces of an infant. Antonie van Leeuwenhoek 117, 50 (2024). https://doi.org/10.1007/s10482-024-01951-3
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DOI: https://doi.org/10.1007/s10482-024-01951-3