Abstract
A gram-stain-positive, aerobic, rod-shaped bacterial strain capable of producing siderophores, named YIM B08730T, was isolated from a soil sample collected from Wumeng Mountain National Nature Reserve, Zhaotong City, Yunnan Province. Growth occurred at 10–45 °C (optimum, 35–40 ℃), pH 7.0–9.0 (optimum, 7.0) and in the presence of 0–5 % (w/v) NaCl (optimum, 0–1 %, w/v). A comparative analysis of the 16S rRNA gene sequence (1558 bp) of strain YIM B08730T showed the highest similarity to Solibacillus isronensis JCM 13838T (96.2 %), followed by Solibacillus silvestris DSM 12223T (96.0 %) and Solibacillus kalamii ISSFR-015T (95.4 %). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and one unidentified lipid. The main respiratory quinone of strain YIM B08730T was menaquinone 7 (MK-7). The major fatty acids were iso-C15:0 and C16:1ω7c alcohol. The digital DNA–DNA hybridization and average nucleotide identity values between strain YIM B08730T and the reference strain S. isronensis JCM 13838T were 24.8 % and 81.2 %, respectively. The G + C content of the genomic DNA was 37.1 mol%. The genome of the novel strain contained genes associated with the production of siderophores, and it also revealed other functional gene clusters involved in plant growth promotion and soil bioremediation. Based on these phenotypic, chemotaxonomic and phylogenetic analyses, strain YIM B08730T is considered to be a novel species of the genus Solibacillus, for which the name Solibacillus ferritrahens sp. nov. is proposed. The type strain is YIM B08730T (= NBRC 116268T = CGMCC 1.60169T).
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Acknowledgements
We would like to thank Aharon Oren (The Hebrew University of Jerusalem) for help about the proposal of bacterial names.
Funding
This work was supported by Major Science and Technology Projects of Yunnan Province (202202AE090015; 202202AE090010; 202002AA100007), Basic Research Project of Yunnan Province (202001BB050025) and the Top Young Talents of “ten thousand talents plan” in Yunnan Province, China.
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X-DL, J-YZ, and L-LL, M-GL and S-KT: Designed the study, carried out the experiments and the data analysis, wrote and revised the manuscript. J-YL, P-WY, S-GL, L-YF, Z-FS, Z-GD: Participated in the data analysis and revised the manuscript. All authors have read and approved the manuscript.
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Liu, XD., Zhao, JY., Li, LL. et al. Solibacillus ferritrahens sp. nov., a novel siderophore-producing bacterium isolated from Wumeng Mountain National Nature Reserve in Yunnan Province. Antonie van Leeuwenhoek 117, 48 (2024). https://doi.org/10.1007/s10482-024-01942-4
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DOI: https://doi.org/10.1007/s10482-024-01942-4