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Nocardioides jiangxiensis sp. nov., a novel actinobacterium isolated from lakeside soil, exhibiting the biosynthesis potential of mycofactocin

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Abstract

A Gram-stain-positive, rod-shaped, non-spore-forming and non-motile bacterium, designated WY-20T, was isolated from a lakeside soil sample collected in Jiangxi Province, PR China. Growth was observed at 20–42 °C (optimum 30 °C), pH 5.0–8.0 (optimum pH 7.0) and salinity of 0–3.0% (w/v; optimum 0.5%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain WY-20T belongs to the genus Nocardioides and showed the highest sequence similarity (98.1%) to N. phosphati WYH11-7T, followed by N. cavernaquae K1W22B-1T (97.8%), N. marmoriterrae JOS5-1T (97.2%) and N. jensenii NBRC 14755T (97.1%). The average nucleotide identity and digital DNA–DNA hybridization values between strains WY-20T and N. phosphati WYH11-7T were 83.5% and 26.2%, respectively. The predominant fatty acids (≥ 10% of the total fatty acids) were C18:1ω9c, C17:0, C16:0, summed feature 8 (C18:1ω7c and/or C18: 1ω6c) and C17:1ω9c. The major menaquinone was MK-8 (H4). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. Based on phenotypic, genotypic and phylogenetic pieces of evidence, strain WY-20T represents a novel species in the genus Nocardioides, for which the name Nocardioides jiangxiensis sp. nov. is proposed. The type strain is WY-20T (= GDMCC 4.317T = KACC 23379T).

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Data availability

The 16S rRNA gene sequence and the whole-genome shotgun sequence of strain WY-20T have been deposited in GenBank/EMBL/DDBJ under the accession numbers OQ781844 and JAUQTA000000000, respectively.

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Funding

This work was supported by the Natural Science Foundation of Jiangxi Province (20224BAB215005), the National Natural Science Foundation of China (31960014, 31660019) and the Special fund for science and technology program of Jiangsu province (BM2022019).

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Authors and Affiliations

  1. College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, Jiangxi, People’s Republic of China

    Yingying Wan, Tianli Sun, Guangwen Huang, Bance Tu, Mingzhu Huang, Xuelan Chen & Bin Liu

  2. Agricultural Microbial Resources Protection and Germplasm Innovation and Utilization Center of Jiangsu Province, Nanjing, 210095, Jiangsu, People’s Republic of China

    Jian He

  3. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China

    Bin Liu & Jian He

Authors
  1. Yingying Wan
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  2. Tianli Sun
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  3. Guangwen Huang
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  4. Bance Tu
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  5. Mingzhu Huang
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  6. Xuelan Chen
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  7. Bin Liu
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  8. Jian He
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Contributions

Conceptualization, funding acquisition, and supervision: XC, BL; Laboratory work, data analysis and writing-original draft: YW, BL; Writing-review and editing: TS, GH, BT, MH, JH. All authors read and approved the final manuscript.

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Correspondence to Xuelan Chen or Bin Liu.

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The GenBank accession number for the 16S rRNA gene sequence of strain WY-20T is OQ781844. The whole-genome shotgun project of strain WY-20T has been deposited into DDBJ/EMBL/GenBank under the accession number JAUQTA000000000.

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Wan, Y., Sun, T., Huang, G. et al. Nocardioides jiangxiensis sp. nov., a novel actinobacterium isolated from lakeside soil, exhibiting the biosynthesis potential of mycofactocin. Antonie van Leeuwenhoek 117, 18 (2024). https://doi.org/10.1007/s10482-023-01910-4

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