Abstract
During our studies on the microorganism diversity from air of manufacturing shop in a pharmaceutical factory in Shandong province, China, a Gram-stain-positive, aerobic, cocci-shaped bacterium, designated LY-0111T, was isolated from a settling dish. Strain LY-0111T grew at temperature of 10–42 °C (optimum 35 °C), pH of 5.0–10.0 (optimum pH 7.0) and NaCl concentration of 1–12% (optimum 0.5–3%, w/v). Based on the 16S rRNA gene sequence analysis, the strain shared the highest sequence similarities to Nesterenkonia halophila YIM 70179T (96.2%), and was placed within the radiation of Nesterenkonia species in the phylogenetic trees. The genome of the isolate was sequenced, which comprised 2,931,270 bp with G + C content of 66.5%. A supermatrix tree based on the gene set bac120 indicated that LY-0111T was close related to Nesterenkonia xinjiangensis YIM 70097T (16S rRNA gene sequence similarity 95.3%). Chemotaxonomic analysis indicated that the main respiratory quinones were MK-7, MK-8, and MK-9, the predominant cellular fatty acids were anteiso-C15:0 and iso-C15:0, and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. According to the phenotypic, chemotaxonomic and phylogenetic features, strain LY-0111T is considered to represent a novel species, for which the name Nesterenkonia aerolata sp. nov. is proposed. The type strain is LY-0111T (= JCM 36375T = GDMCC 1.3945T). In addition, Nesterenkonia jeotgali was proposed as a later synonym of Nesterenkonia sandarakina, according to the ANI (96.8%) and dDDH (72.9%) analysis between them.
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Data availability
Most of the data supporting the conclusions of this article are included within the article and its additional files. The genome datasets and the 16S rRNA gene sequence of Nesterenkonia aerolata, sp. nov. LY0111T generated during the current study are available in the GenBank repository under accession number JAVKGR000000000 and OR564096, respectively. The genome datasets of Nesterenkonia flava CCTCC AB 207010T generated during the current study are available in the GenBank repository under accession number JAVKGT000000000. Other datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
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Funding
This research was supported by grants from the Science and Technology Commission of Shanghai Municipality (22142201600), the Standard improvement project of the Chinese Pharmacopoeia Commission (2023Y36), the opening fund of NMPA Key Laboratory for Testing Technology of Pharmaceutical Microbiology (2022-WSW-01).
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MHS and DFZ designed research and project outline. MHS, WLM, and WH performed isolation, deposition and polyphasic taxonomy. HCW, NZ and DFZ performed genome analysis. MHS and WLM drafted the manuscript. DFZ, SW, and YLF revised the manuscript. All authors read and approved the final manuscript.
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Song, MH., Ma, WL., Zhang, N. et al. Description of Nesterenkonia aerolata sp. nov., an actinobacterium isolated from air of manufacturing shop in a pharmaceutical factory. Antonie van Leeuwenhoek 117, 8 (2024). https://doi.org/10.1007/s10482-023-01905-1
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DOI: https://doi.org/10.1007/s10482-023-01905-1