Abstract
Mangroves are a challenging ecosystem for the microorganisms that inhabit them, considering they are subjected to stressful conditions such as high and fluctuating salinity. Metagenomic analysis of mangrove soils under contrasting salinity conditions was performed at the mouth of the Ranchera River to the Caribbean Sea in La Guajira, Colombia, using shotgun sequencing and the Illumina Hiseq 2500 platform. Functional gene analysis demonstrated that salinity could influence the abundance of microbial genes involved in osmoprotectant transport, DNA repair, heat shock proteins (HSP), and Quorum Sensing, among others. In total, 135 genes were discovered to be linked to 12 pathways. Thirty-four genes out of 10 pathways had statistical differences for a p-value and FDR < 0.05. UvrA and uvrB (nucleotide excision repair), groEL (HSP), and secA (bacterial secretion system) genes were the most abundant and were enriched by high salinity. The results of this study showed the prevalence of diverse genetic mechanisms that bacteria use as a response to survive in the challenging mangrove, as well as the presence of various genes that are recruited in order to maintain bacterial homeostasis under conditions of high salinity.
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We would like to express our gratitude to MINCIENCIAS, Universidad Antonio Nariño, and Universidad Nacional de Colombia for their funding and technical support during the project execution.
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This study was funded by MINCIENCIAS, Universidad Antonio Nariño, and Universidad Nacional de Colombia through grant number code 123365944129 and contract FP44842-529-2014 within the BIOS' call of the Colombian state for research in biodiversity of strategic ecosystems for the country.
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Sepúlveda-Correa, A., Monsalve, L., Polania, J. et al. Effect of salinity on genes involved in the stress response in mangrove soils. Antonie van Leeuwenhoek 116, 1171–1184 (2023). https://doi.org/10.1007/s10482-023-01856-7
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DOI: https://doi.org/10.1007/s10482-023-01856-7