Abstract
An anaerobic, Gram-staining-negative, rod-shaped, Fe(III)-reducing strain, designated SG189T, was isolated from paddy soil in Fujian Province, China. Growth occurred at 20–35 ℃ (optimum 30 ℃), pH 6.5–8.0 (optimum 7.0) and 0–0.2% (w/v) NaCl (optimum 0%). The strain SG189T showed the highest 16S rRNA sequences similarities to the type strains of Geothrix fermentans DSM 14018T (98.9%), “Geothrix terrae” SG184T (99.0%) and “Geothrix alkalitolerans” SG263T (99.3%). ANI and dDDH values between strain SG189T and the most closely related Geothrix species were 86.5–87.1% and 31.5–32.9%, which lower than the cut-off values (ANI 95–96% and dDDH 70%) for prokaryotic species delineation. Further, genome-based phylogenomic trees constructed using 81 core genes (UBCG2) and 120 conserved genes (GTDB) showed that strain SG189T formed a clade with members of the genus Geothrix. The menaquinone was shown to be MK-8, and the major fatty acids were iso-C15:0 and iso-C13:0 3OH. The genomic DNA G + C content was 68.2%. Additionally, we found that strain SG189T possessed ability to reduce ferric iron, and strain SG189T could reduce 10 mM of ferric citrate in 10 days with lactate as the sole electron donor. Based on the observed physiological and biochemical properties, chemotaxonomic characteristics, ANI and dDDH values, SG189T represents a novel species of the genus Geothrix, for which the name Geothrix oryzisoli sp. nov. is proposed. The type strain is SG189T (= GDMCC 1.3408T = JCM 39324T).
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Abbreviations
- dDDH:
-
Digital DNA-DNA hybridization
- ANI:
-
Average nucleotide identity
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Acknowledgements
We are very grateful to Prof. A. Oren for his kindly help and advice on nomenclature.
Funding
This work was supported by the National Natural Science Foundation of China (Grant: 41925028 and U21A20295) and Natural Science Foundation of Fujian Province (Grant: 2020J02015).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Shuang Han, Rong Tang, Shang Yang and Cheng-Jie Xie. The first draft of the manuscript was written by Shuang Han and all authors commented on previous versions of the manuscript. Guo-Hong Liu and Shun-Gui Zhou designed and supervised the study and edited the original draft. Christopher Rensing and Manik Prabhu Narsing Rao revised the manuscript. All authors read and approved the final manuscript.
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Han, S., Tang, R., Yang, S. et al. Geothrix oryzisoli sp. nov., a ferric iron-reducing bacterium isolated from paddy soil. Antonie van Leeuwenhoek 116, 477–486 (2023). https://doi.org/10.1007/s10482-023-01817-0
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DOI: https://doi.org/10.1007/s10482-023-01817-0