Abstract
Three novel facultatively methylotrophic bacteria, strains 3CT, 1A, 8P, were isolated from activated sludges. The isolates were aerobic, Gram-stain-negative, non-motile, non-spore forming rods multiplying by binary fission. The predominant polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethylethanolamine, phosphatidylmonomethylethanolamine, and diphosphatidylglycerol. The major fatty acids of cells were С18:1ω7c, C19:0ω8c cyclo and C16:0. Levels of 16S rRNA gene similarity indicates that the closely relatives are representatives of the genera Starkeya, Ancylobacter, Angulomicrobium and Methylorhabdus (96.4–99.4%). Genomic comparisons of 3CT and its closest relatives, S. novella DSM 506T and S. koreensis Jip08T, shared 87.3 and 86.8% nucleotide identity and 28.3 and 26.8% digital DNA–DNA hybridization values, respectively. The average amino acid identities between the strain 3CT and representatives of Starkeya, Ancylobacter and Angulomicrobium were in the range of 75.6–84.3%, which combines these strains into a single genus and gives rise to their reclassification. Based on polyphasic analyses, the strains 3CT, 1A, 8P represents a novel species of the genus Ancylobacter, for which the name Ancylobacter moscoviensis sp. nov. is proposed. The type strain is 3CT (= VKM B-3218T = KCTC 62336T). Furthermore, we also suggested the reclassification of Starkeya novella as Ancylobacter novellus comb. nov., Starkeya koreensis as Ancylobacter koreensis comb. nov., Angulomicrobium tetraedrale as Ancylobacter tetraedralis comb. nov., Angulomicrobium amanitiforme as Ancylobacter amanitiformis comb. nov. and Methylorhabdus multivorans as Ancylobacter multivorans comb. nov. with the emended description of the genus Ancylobacter.
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Acknowledgements
We thank Professor Aharon Oren for his advice regarding the nomenclature. We are grateful to N.V. Prisyazhnaya (All-Russian Collection of Microorganisms, IBPM RAS) for MALDI analysis.
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This study was supported by the Ministry of Science and Higher Education of the Russian Federation, grant agreement № 075-15-2021-1051 and the National Key R&D Program of China (2017YFC1602002).
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N.D.: isolation of cultures, formal analysis, data curation, original draft preparation, review and editing, visualization; A.Ch.: formal analysis; V.K.: genome sequencing of the type strain, the section on genome features; D.G.: genome sequencing of the type strain, the sections on bioinformatic phylogenomic analyses and genome features, review and editing; N.A.: original draft preparation, data curation, review and editing; W.S.: genome sequence and data analyses of Ancylobacter spp.; L.W.: genome of sequence and data analyses; E.K.: formal analysis, data curation, original draft preparation, review and editing.
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Doronina, N.V., Chemodurova, A.A., Grouzdev, D.S. et al. Ancylobacter moscoviensis sp. nov., novel facultatively methylotrophic bacteria from activated sludge and the reclassification of Starkeya novella (Starkey 1934) Kelly et al. 2000 as Ancylobacter novellus comb. nov., Starkeya koreensis Im et al. 2006 as Ancylobacter koreensis comb.nov., Angulomicrobium tetraedrale Vasil'eva et al. 1986 as Ancylobacter tetraedralis comb. nov., Angulomicrobium amanitiforme Fritz et al. 2004 as Ancylobacter amanitiformis comb. nov., and Methylorhabdus multivorans Doronina et al. 1996 as Ancylobacter multivorans comb. nov., and emended description of the genus Ancylobacter. Antonie van Leeuwenhoek 116, 153–170 (2023). https://doi.org/10.1007/s10482-022-01788-8
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DOI: https://doi.org/10.1007/s10482-022-01788-8