Abstract
A Gram-stain-positive, orange-pigmented, rod-shaped and flagellated bacterial strain T12T was isolated from wetland soil in Kunyu Mountain Wetland in Yantai, China. The strain was able to grow at 15–40 °C (optimum 37 °C), at 0.0–9.0% NaCl (optimum 2%, w/v) and at pH 5.5–9.0 (optimum 8.5). A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain T12T is a member of the family Planococcaceae, sharing 97.6% and 97.1% sequence similarity with the type strains of Jeotgalibacillus salarius and Jeotgalibacillus marinus, respectively. Genome-based analyses revealed a genome size of 3,506,682 bp and a DNA G + C content of 43.7%. Besides, the genome sequence led to 55.0–74.6% average amino acid identity values and 67.8–74.7% average nucleotide identity values between strain T12T and the current closest relatives. Digital DNA-DNA hybridization of strain T12T with the type strains of Jeotgalibacillus proteolyticus and J. marinus demonstrated 19.0% and 20.3% relatedness, respectively. The chemotaxonomic analysis showed that the sole quinone was MK-7. The predominant cellular fatty acids were iso-C15:0, anteiso-C15:0, C16:1ω7c alcohol and iso-C14:0. The polar lipids consisted of an unidentified aminolipid, phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. Based on the polyphasic characterization, strain T12T is considered to represent a novel species, for which the name Jeotgalibacillus aurantiacus sp. nov. is proposed. The type strain is T12T (= KCTC 43296 T = MCCC 1K07171T).
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Data availability
All data generated or analysed during this study are included in this published article, its supplementary information files and GenBank/EMBL/DDBJ. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain T12T is MW527064 and the number for the whole genome sequence is JACNMS000000000. Supplementary figures and Supplementary tables are available with the online version of this paper.
Abbreviations
- AAI:
-
Average amino acid identity
- AL:
-
Aminolipid
- ANI:
-
Average nucleotide identity
- BGCs:
-
Biosynthetic gene clusters
- COG:
-
Cluster of orthologous groups
- dDDH:
-
Digital DNA-DNA hybridization
- DPG:
-
Diphosphatidylglycerol
- GGDC:
-
Genome-to-genome distance calculator
- HPLC:
-
High performance liquid chromatography
- KCTC:
-
Korean collection for type cultures
- KEGG:
-
Kyoto encyclopedia of genes and genomes
- MA:
-
Marine agar 2216
- MB:
-
Marine broth 2216
- MEGA:
-
Molecular evolutionary genetics analysis
- MIDI:
-
Microbial identification system
- MCCC:
-
Marine culture collection of China
- PC:
-
Phosphatidylcholine
- PG:
-
Phosphatidylglycerol
- RAST:
-
Rapid annotations using subsystems technology
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Acknowledgements
We are very grateful to Prof. Aharon Oren for his advice on naming the species.
Funding
This work was supported by the National Natural Science Foundation of China (31700116), the Natural Science Foundation of Shandong Province (ZR2017MC019), the China Postdoctoral Science Foundation (2017M62218), and the Key Science and Technology Program of Weihai (1070413421511).
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HNJ drafted the manuscript. BXW, STY, HNJ and YM performed isolation, deposition and identification. STY, BXW and MJZ performed the genome analysis. STY and YXZ revised the manuscript. YXZ designed all the experiments and supervised the manuscript.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain T12T is MW527064 and the number for the whole genome sequence is JACNMS000000000 (assembly accession GCA_020595125.1).
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Jiang, HN., Yun, ST., Wang, BX. et al. Jeotgalibacillus aurantiacus sp. nov., a novel orange-pigmented species with a carotenoid biosynthetic gene cluster, isolated from wetland soil. Antonie van Leeuwenhoek 115, 773–782 (2022). https://doi.org/10.1007/s10482-022-01731-x
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DOI: https://doi.org/10.1007/s10482-022-01731-x