Abstract
A Gram-stain-negative, wheat, rod-shaped, non-motile, non-spore forming, and facultatively anaerobic bacterium strain, designated as PIT, was isolated from saline silt samples collected in saltern in Yantai, Shandong, China. Growth was observed within the ranges 4–45 °C (optimally at 33 °C), pH 6.0–9.0 (optimally at pH 7.0) and 1.0–11.0% NaCl (optimally at 3.0%, w/v). Strain PIT showed highest 16S rRNA gene sequence similarity to Kangiella sediminilitoris BB-Mw22T (98.3%) and Kangiella taiwanensis KT1T (98.3%). The major cellular fatty acids (> 10% of the total fatty acids) were iso-C15:0 (52.7%) and summed featured 9 (iso-C17:1ω9c/C16:0 10-methyl, 11.8%). The major polar lipids identified were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and phosphatidylglycerol. The major respiratory isoprenoid quinone was Q-8. The G + C content of the genomic DNA was 45.8%. Average Nucleotide Identity values between whole genome sequences of strain PIT and next related type strains supported the novel species status. Based on physiological, biochemical, chemotaxonomic characteristics and genomic analysis, strain PIT is considered to represent a novel species within the genus Kangiella, for which the name Kangiella shandongensis sp. nov. is proposed. The type strain is PIT (= KCTC 82509 T = MCCC 1K04352T).
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Abbreviations
- AAI:
-
Average amino acid identity
- ANI:
-
Average nucleotide identity
- COG:
-
Cluster of orthologous groups
- dDDH:
-
Digital DNA-DNA hybridization
- DPG:
-
Diphosphatidylglycerol
- GGDC:
-
Genome-to-genome distance calculator
- HPLC:
-
High performance liquid chromatography
- KCTC:
-
Korean collection for type cultures
- KEGG:
-
Kyoto encyclopedia of genes and genomes
- MA:
-
Marine agar 2216
- MB:
-
Marine broth 2216
- MEGA:
-
Molecular evolutionary genetics analysis
- MCCC:
-
Marine culture collection of china
- PE:
-
Phosphatidylethanolamine
- PG:
-
Phosphatidylglycerol
- PME:
-
Phosphatidylmonomethylethanolamine
- VFDB:
-
Virulence factors of pathogenic bacteria
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (31700116), the Natural Science Foundation of Shandong Province (ZR2017MC019), the China Postdoctoral Science Foundation (2017M62218), and the Key Science and Technology Program of Weihai (1070413421511).
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LYP wrote the manuscript and performed the research. HNJ, XXL and YM performed isolation, deposition, and identification. LYP and SKG performed genome analysis. YXZ contributed to study design. YXZ and RY revised the paper. All authors read and approved the final manuscript.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain PIT is MW407073 and the number for the whole genome sequence is JACNML000000000.
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Peng, LY., Yin, R., Gao, SK. et al. Kangiella shandongensis sp. nov., a novel species isolated from saltern in Yantai, China. Antonie van Leeuwenhoek 115, 33–40 (2022). https://doi.org/10.1007/s10482-021-01682-9
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DOI: https://doi.org/10.1007/s10482-021-01682-9