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Pacificispira spongiicola gen. nov., sp. nov., a nitrate-reducing bacterium isolated from tropical western Pacific

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Abstract

The Gram-stain-negative, strictly aerobic, curved-to-spiral rod-shaped bacterial strain, designated KN72T, was isolated from the Caroline Seamounts in the Pacific Ocean. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain KN72T was a member of the family Rhodospirillaceae and formed a distinct lineage. Strain KN72T contained ubiquinone-10 as the major respiratory quinone. The polar lipid profiles contained phosphatidylethanolamine, phosphatidylglycerol, one aminolipid and three phospholipids. The predominant cellular fatty acids were C16:0 and summed feature 8 (comprising C18:1ω7c/C18:1ω6c). The strain KN72T displayed highest 16S rRNA gene sequence similarities with Hwanghaeella grinnelliae Gri0909T (92.3%), Marivibrio halodurans ZB80T (91.0%) and Aestuariispira insulae AH-MY2T (90.1%). The DNA G+C content of strain KN72T was 61.1%. Collectively, based on phenotypic, chemotaxonomic, phylogenetic and genomic evidence presented, strain KN72T represents a novel species of a novel genus of the family Rhodospirillaceae, for which the name Pacificispira spongiicola gen. nov., sp. nov. is proposed. The type strain is KN72T (= CGMCC 1.17142T = KCTC 72429T).

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Availability of data and materials

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request. All data generated or analysed during this study are included in this published article [and its supplementary information files].

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Funding

This study was supported by the Science & Technology Basic Resources Investigation Program of China (2017FY100804, awarded to Dechao Zhang), National Natural Science Foundation of China (31670002, awarded to Dechao Zhang), and the Senior User Project of RV KEXUE (KEXUE2019G09, awarded to Dechao Zhang).

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Wenxuan He & Dechao Zhang

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Wenxuan He & Dechao Zhang

  3. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China

    Yangjie Li

  4. Center for Ocean Mega-Science, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China

    Dechao Zhang

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  1. Wenxuan He
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  2. Yangjie Li
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Contributions

ZDC designed this research, HWX performed strain characterization, phenotypic determination and chemotaxonomic analysis, LYJ performed the genomic and phylogenetic analysis, HWX and LYJ drafted the manuscript. ZDC supervised the study and contributed to text preparation and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Dechao Zhang.

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He, W., Li, Y. & Zhang, D. Pacificispira spongiicola gen. nov., sp. nov., a nitrate-reducing bacterium isolated from tropical western Pacific. Antonie van Leeuwenhoek 114, 2083–2090 (2021). https://doi.org/10.1007/s10482-021-01662-z

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  • DOI: https://doi.org/10.1007/s10482-021-01662-z

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