Abstract
Microaerophilic veils of swimming microorganisms form at oxic-anoxic interfaces, mostly described in sediments where sulfide from below meets oxygen diffusing in from the water phase. However, microaerophilic veils form even when these gradients do not overlap, for example when cable bacteria activity leads to a suboxic zone. This suggests that veil microorganisms can use electron donors other than sulfide. Here we describe the extraction of microorganisms from a microaerophilic veil that formed in cable-bacteria-enriched freshwater sediment using a glass capillary, and the subsequent isolation of a motile, microaerophilic, organoheterotrophic bacterium, strain R2-JLT, unable to oxidize sulfide. Based on phenotypic, phylogenetic, and genomic comparison, we propose strain R2-JLT as a novel Phyllobacterium species, P. calauticae sp. nov.. The type strain is R2-JLT (= LMG 32286T = DSM 112555T). This novel isolate confirms that a wider variety of electron donors, including organic compounds, can fuel the activity of microorganisms in microaerophilic veils.
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P. calauticae R2-JLT is available from BCCM/LMG Gent, Belgium (LMG 32286T) and DSMZ, Braunschweig, Germany (DSM 112555T). The draft genome sequence is available from NCBI under the accession number JAGENB000000000.
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Acknowledgements
We thank Susanne Nielsen and Lars B. Pedersen for their work and support in the molecular and microbiological laboratories; Pia Bomholt Jensen for excellent SEM images; and Ronny Mario Baaske for care of the cable bacteria enrichments. This research was supported by the Danish National Research Foundation (DNRF136) and the Carlsberg Foundation (CF19-0666)
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This research was supported by the Danish National Research Foundation (DNRF136) and the Carlsberg Foundation (CF19-0666).
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JL, JB, AS, and IM conceived and designed the study; JL and AS performed research; JL analysed data; JL, JB, and IM contributed new methods or models; JL, AS, and IM wrote the paper; all authors have read and approved the final manuscript.
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Lustermans, J.J.M., Bjerg, J.J., Schramm, A. et al. Phyllobacterium calauticae sp. nov. isolated from a microaerophilic veil transversed by cable bacteria in freshwater sediment. Antonie van Leeuwenhoek 114, 1877–1887 (2021). https://doi.org/10.1007/s10482-021-01647-y
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DOI: https://doi.org/10.1007/s10482-021-01647-y