Abstract
A Gram-stain-negative, aerobic, rod-shaped, non-gliding and non-motile bacterium designated as N1E253T, was isolated from marine sediments collected from the coast of Weihai, PR China. N1E253T was found to grow at pH 7.0–9.0 (optimum, pH 7.5), 15–37 °C (optimum, 30 °C) in the presence of 1.5–5.0% (w/v) NaCl (optimum, 3.0%). The major polar lipids of strain N1E253T were phosphatidylethanolamine, diphosphatidylglycerol and one unidentified phospholipid. The sole respiratory quinone was MK-9. The major cellular fatty acids (> 10.0%) were iso-C14: 0, C16: 0 and Summed Feature 3 (C16:1 ω7c and/or C16:1 ω6c). The result of the 16S rRNA gene sequence analysis confirmed the affiliation of this novel isolate to the family Verrucomicrobiaceae, with Persicirhabdus sediminis KCTC 22039 T being its closest relative with 92.1% sequence similarity. Genome sequencing revealed a genome size of 5,073,947 bp, DNA G + C content of 52.0% and two protein-coding genes related to the resistance of fluoroquinolones. Based on physiological, genomic, biochemical and chemotaxonomic characteristics, we propose that strain N1E253T represents a novel species of a novel genus within the family Verrucomicrobiaceae, for which the name Oceaniferula marina gen. nov., sp. nov. is proposed. The type strain is N1E253T (= KCTC 72800 T = MCCC 1H00405T).
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Data Availability
The GenBank accession numbers for the 16S rRNA gene sequence and the draft whole genome data of strain N1E253T are MN897724 and JACBAZ000000000, respectively.
Abbreviations
- KCTC:
-
Korean collection for type cultures
- MCCC:
-
Marine culture collection of China
- MEGA:
-
Molecular evolutionary genetics analysis
- MA:
-
Marine agar 2216
- MB:
-
Marine broth 2216
- HPLC:
-
High performance liquid chromatography
- TLC:
-
Thin-layer chromatography
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Acknowledgements
The scanning electron microscopy was supported by the Physical-Chemical Materials Analytical and Testing Center of Shandong University at Weihai. The GenBank accession number for the 16S rRNA gene sequences of strain N1E253T is MN897724. The draft genome of strain N1E253T has been deposited in GenBank under the accession number JACBAZ000000000.
Funding
This work was supported by the National Natural Science Foundation of China (31770002) and the National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700).
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Jin, CB., Feng, X., Zou, QH. et al. Oceaniferula marina gen.nov., sp.nov., an anti-fluoroquinolone bacterium isolated from marine sediment. Antonie van Leeuwenhoek 114, 1855–1865 (2021). https://doi.org/10.1007/s10482-021-01645-0
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DOI: https://doi.org/10.1007/s10482-021-01645-0