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Citrobacter arsenatis sp. nov., an arsenate-reducing bacterium isolated from freshwater sediment

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Abstract

A novel arsenate-reducing bacterium, LY-1T, was isolated from freshwater sediment in Huangshi, China. Morphological analysis indicated that the cells were shaped like rods and were gram-negative. The major fatty acids (> 10%) were C16:0, summed feature 3 (C16:1 ω7c, C16:1 ω6c) and summed feature 8 (C18:1 ω7c, C18:1 ω6c). An assessment of the phylogeny based on 16S rRNA gene sequences indicated that the strain LY-1T belonged to the genus Citrobacter, while further analysis based on the recN gene indicated that LY-1T occupies a distinct phylogenetic niche within the Citrobacter genus. Moreover, average nucleotide identity and digital DNA-DNA hybridization between the strain LY-1T and the type strains of closely related species of the genus Citrobacter (C. europaeus, C. brakii, C. portucalensis, C. freundii, C. werkmanii, C. cronae, C. youngae, C. pasteurii, C. tructae, C. gillenii, and C. murliniae) were 85.8–93.8% and 31.2–56.9%, respectively. In addition, the LY-1T strain’s capacity to metabolize various compounds and its characteristic G + C content of 51.9% were also distinct from other species of the Citrobacter genus. These discriminatory features cumulatively indicate the LY-1T strain as a new species within the Citrobacter genus. We propose the species name Citrobacter arsenatis for this new species, with LY-1T (= CCTCC AB 2019169T = KCTC 72440T) as the type strain.

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Acknowledgements

This work was financially supported by Major Program of Technical Innovation Foundation of Hubei Province (2016ACA176), Research Foundation for Talented Scholars of HBPU (16xjz03R), Innovation Training Program in Hubei Province (201710920043), Science and Technology Research Project of Hubei Provincial Department of Education (B2016272), Science and Technology Research Project of HBPU (19XJK01Y) and Open Fund Project of Hubei Key Laboratory of Mine Environmental Pollution Control and Remediation (2013105)

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Authors and Affiliations

  1. School of Resource and Environment Science, Wuhan University, Wuhan, China

    Hanlin Wang & Haobo Hou

  2. School of Environmental Science and Engineering, Hubei Key Laboratory of Mine Environmental Pollution Control and Remediation, Hubei Polytechnic University, Huangshi, China

    Hanlin Wang & Jiexun Huang

Authors
  1. Hanlin Wang
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  2. Haobo Hou
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  3. Jiexun Huang
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Contributions

JH and HH conceived the study. HW performed the experiments. HW and JH analyzed the data, and HW, JH and HH drafted the manuscript. All authors have read and approved the manuscript.

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Correspondence to Haobo Hou or Jiexun Huang.

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I certify that this manuscript is original and has not been published and will not be submitted elsewhere for publication while being considered by Haobo Hou. And the study is not split up into several parts to increase the quantity of submissions and submitted to various journals or to one journal over time. No data have been fabricated or manipulated (including images) to support your conclusions. No data, text, or theories by others are presented as if they were our own. The submission has been received explicitly from all co-authors. And authors whose names appear on the submission have contributed sufficiently to the scientific work and therefore share collective responsibility and accountability for the results.

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Wang, H., Hou, H. & Huang, J. Citrobacter arsenatis sp. nov., an arsenate-reducing bacterium isolated from freshwater sediment. Antonie van Leeuwenhoek 114, 1285–1292 (2021). https://doi.org/10.1007/s10482-021-01601-y

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