Abstract
A set of four strains representing a novel basidiomycetous yeast species Rhodotorula sampaioana f. a., sp. nov. were isolated from two different habitats, subsurface waters of Lake Negra in Argentina, and the gut of a xylophagous termite in India. Phylogenetic analyses of LSU and ITS sequences showed that they belonged to the genus Rhodotorula of the order Sporidiobolales (subphylum Pucciniomycotina) and the closest known relative being R. kratochvilovae. The new species differed from R. kratochvilovae CBS 7436 (AF071436, AF444520) by nine nucleotide substitutions and one deletion (1.7 % sequence variation) in a 593 bp D1/D2 region, and by five nucleotide substitutions and three deletions (1.3 %) in a 592 bp ITS region, respectively. Several morphological and physiological differences were also observed between R. kratochvilovae and the strains obtained during this study. These data support the proposal of Rhodotorula sampaioana as a novel species, with CRUB 1124 as the holotype, CBS 10798 as ex-type, and NFCCI 4872 as an additional strain. The GenBank accession numbers of the LSU and ITS sequences of Rhodotorula sampaioana f. a., sp. nov. are EF595748 and MW879331. The MycoBank number is MB 838533.
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All data generated or analysed during this study are included in this published article [and its supplementary information files]. The strains used in the present study are available at the Westerdijk Fungal Biodiversity Institute Utrecht, The Netherlands, and the National Fungal Culture Collection of India (NFCCI), India.
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Acknowledgements
We duly acknowledge the help extended by Dr Karthick Balasubramanian and Mr Rameshwar Avchar from Agharkar Research Institute, Pune, India. ST is thankful to the University Grants Commission, Delhi (India) for senior research fellowship [827/(CSIR-UGC NET DEC.2017)]. DL thanks the authorities of Administración de Parques Nacionales (Argentina) for providing sampling permits. This work was partially supported by CONICET (PIP11220130100392CO), and Universidad Nacional del Comahue (B199).
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This study was supported by financial aid from Universidad Nacional del Comahue (Project B199) and CONICET (Project PIP424).
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Snigdha Tiwari: Sampling, Methodology, Formal analysis and Investigation, Data Curation, Writing- Original draft. Abhishek Baghela: Methodology, Data Curation, Resources, Supervision, Project administration, Writing– review and editing. Diego Libkind: Sampling, Methodology, Formal analysis and Investigation, Data Curation, Writing– Original draft, review and editing.
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Tiwari, S., Baghela, A. & Libkind, D. Rhodotorula sampaioana f.a., sp. nov., a novel red yeast of the order Sporidiobolales isolated from Argentina and India. Antonie van Leeuwenhoek 114, 1237–1244 (2021). https://doi.org/10.1007/s10482-021-01597-5
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DOI: https://doi.org/10.1007/s10482-021-01597-5