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Marinobacterium alkalitolerans sp. nov., with nitrate reductase and urease activity isolated from green algal mat collected from a solar saltern

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Abstract

A novel Gram-staining-negative, rod-shaped, 0.6–0.8 µm wide and 2.0–3.0 µm in length, motile bacterium designated strain AK62T, was isolated from the green algal mat collected from saltpan, Kakinada, Andhra Pradesh, India. Colonies on ZMA were circular, off-white, shiny, moist, translucent, 1–2 mm in diameter, flat, with an entire margin. The major fatty acids include C16:0, C18:1 ω7c, and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c and/or iso-C14:0 3-OH). Polar lipids include diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, three unidentified phospholipids, and one unidentified lipid. Polyamine includes Spermidine. The DNA G + C content of the strain AK62T was 58.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain AK62T was closely related to the type strains Marinobacterium sediminicola, Marinobacterium coralli and Marinobacterium stanieri with a pair-wise sequence similarity of 96.9, 96.6 and 96.6%, respectively, forming a distinct branch within the genus Marinobacterium and clustered with M. stanieri, M. sediminicola, M. coralli and M. maritimum cluster. Strain AK62T shares average nucleotide identity (ANIb, based on BLAST) of 78.44, 76.69, and 76.95% with M. sediminicola CGMCC 1.7287T, M. stanieri DSM 7027T, and Marinobacterium halophilum Mano11T respectively. Based on the observed phenotypic, chemotaxonomic characteristics, and phylogenetic analysis, strain AK62T is described in this study as a novel species in the genus Marinobacterium, for which the name Marinobacterium alkalitolerans sp. nov. is proposed. The type strain of M. alkalitolerans is AK62T (= MTCC 12102T = JCM 31159T = KCTC 52667T).

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Data availability

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain AK62T is LN558833. The GenBank/EMBL/DDBJ accession number for the whole genome of strain AK62T is JACVEW000000000. All authors contributed equally to the work.

Availability of data and materials

The data and cultures are available.

Code availability

Here is a software application or custom code developed.

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Acknowledgements

The authors thank the Directors of, National Institute of Oceanography and Institute of Microbial Technology. We thank the Council of Scientific and Industrial Research (CSIR) and the Department of Biotechnology, Government of India, for financial assistance. TNRS is thankful for Projects OLP1710 and GAP 3195 for providing facilities and funding, respectively. We also thank Mr Deepak Bhatt for technical assistance in sequencing the 16S rRNA gene. GS thanks CSIR for the research fellowship. The NIO contribution number is 6725.

Funding

The work is supported by the funds received from the Institutional Project OLP1710 and Ministry of Earth Sciences Project GAP 3195.

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Authors and Affiliations

  1. CSIR-National Institute of Oceanography, Regional Centre, 176, Lawsons Bay Colony, Visakhapatnam, 530017, India

    Naga Radha Srinivas Tanuku, Sudharani Pydi, Swarnaprava Behera & Sampath Kumar Ganta

  2. MTCC-Microbial Type Culture Collection and Gene Bank, CSIR-Institute of Microbial Technology, Chandigarh, 160036, India

    Anil Kumar Pinnaka, Vasundhara Gupta, Gunjan Vasudeva & Nishant Kashyap

  3. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    Anil Kumar Pinnaka, Naga Radha Srinivas Tanuku & Swarnaprava Behera

Authors
  1. Anil Kumar Pinnaka
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  2. Naga Radha Srinivas Tanuku
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  3. Vasundhara Gupta
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  4. Gunjan Vasudeva
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  8. Sampath Kumar Ganta
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Contributions

PAK, TNRS: Conceptualization, designed study, analyzed data, methodology, writing—original draft, Performed research: VG, GV, PSR, NK, SB, GSK.

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Correspondence to Naga Radha Srinivas Tanuku.

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Pinnaka, A.K., Tanuku, N.R.S., Gupta, V. et al. Marinobacterium alkalitolerans sp. nov., with nitrate reductase and urease activity isolated from green algal mat collected from a solar saltern. Antonie van Leeuwenhoek 114, 1117–1130 (2021). https://doi.org/10.1007/s10482-021-01582-y

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