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Hymenobacter negativus sp. nov., bacteria isolated from mountain soil collected in South Korea

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Abstract

Two novel Gram-negative bacterial strains BT442T and BT584 were isolated from dry soil collected in mountains Busan and Guri, Korea during wintertime. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT442T and BT584 both belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia). Strain BT442T was closely related to Hymenobacter soli PB17T (98.0% 16S rRNA gene similarity) and Hymenobacter terrae POA9T (97.6%). No other recognized bacterial species showed more than 97% 16S rRNA gene sequence similarity to strains BT442T. The genome size of strain BT442T was 5,143,362 bp. Bacterial growth was observed at 10–30 °C (optimum 25 °C), pH 6.0–8.0 (optimum pH 6.0) in R2A agar and in the presence up to 1% NaCl. The major cellular fatty acids of strains BT442T and BT584 were iso-C15:0, anteiso-C15:0 and summed feature 3 (C16:1 ω6c / C16:1 ω7c). In addition, their predominant respiratory quinone was MK-7. The major polar lipids of strains BT442T and BT584 were identified to be phosphatidylethanolamine, aminophospholipid, and aminolipid. Based on the biochemical, chemotaxonomic, and phylogenetic analyses, strains BT442T and BT584 are novel bacterial species within the genus Hymenobacter, and the proposed name is Hymenobacter negativus. The strain type of Hymenobacter negativus is BT442T (= KCTC 72902T = NBRC XXXXT).

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The GenBank accession numbers for the 16S rRNA gene sequences of strain BT442T and strain BT584 are MT815535 and MT893355, respectively. The whole genome sequences of strain BT442T and strain BT584 have been deposited in GenBank under accession numbers NZ_JAEDAE000000000 and NZ_JAEDAD000000000, respectively.

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Acknowledgements

This work was supported by a research grant from Seoul Women’s University (2021) and a grant from the National Institute of Biological Resources (NIBR), which was funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002108). We are grateful to Dr. Aharon Oren (The Hebrew University of Jerusalem, Israel) for helping with the etymology.

Funding

This work was supported by a research grant from Seoul Women’s University (2021) and by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002203).

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Authors and Affiliations

  1. Department of Bio and Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 01797, Republic of Korea

    Soohyun Maeng & Myung Kyum Kim

  2. Department of Food and Nutrition, Kookmin University, Seoul, 02707, Republic of Korea

    Yoonjee Chang

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  1. Soohyun Maeng
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  2. Myung Kyum Kim
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  3. Yoonjee Chang
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Conceptualization, funding acquisition, and supervision: MKK; Laboratory work, data analysis and writing-original draft: SM; Writing-review and editing: YC.

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Correspondence to Myung Kyum Kim or Yoonjee Chang.

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Maeng, S., Kim, M.K. & Chang, Y. Hymenobacter negativus sp. nov., bacteria isolated from mountain soil collected in South Korea. Antonie van Leeuwenhoek 114, 1025–1031 (2021). https://doi.org/10.1007/s10482-021-01573-z

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