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Streptomyces polyasparticus sp. nov. isolated from cotton field soil by a medium applied with polyaspartic acid

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Abstract

A novel Streptomyces strain (TRM66268-LWLT) was isolated from cotton field soil by a medium supplied with polyaspartic acid (PASP) at Alar, Xinjiang, Northwest PR China, and characterized using a polyphasic taxonomic approach. The strain was found to degrade PASP, and grow well on the medium to take PASP as the sole carbon source. The TRM66268-LWLT fermentation broth was applied to the surface of PASP, and there were pores on the surface of PASP after a period of time. The strain was observed to be Gram-stain-positive and to form greyish-white aerial mycelia that differentiated into straight spore chains with round spores. The whole-cell sugar pattern of TRM 66268-LWLT consisted of ribose, mannose and arabinose, and the principal phospholipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside, phosphatidylinositol and two undetermined polar lipids. The predominant menaquinones were MK-7, MK-7(H4), MK-9(H8), MK-10(H6). The diagnostic cell wall amino acid was identified as LL-diaminopimelic acid. The G+C content of strain TRM66268-LWLT was 70.11 mol%. The average nucleotide identity value between strain TRM66268-LWLT and the phylogenetically related strain Streptomyces indicus IH32-1T was calculated to be 85.49%. The digital DNA-DNA hybridization value between them was 30.40%. A multilocus sequence analysis of five house-keeping genes (atpD, gyrB, rpoB, recA and trpB) also illustrated that strain TRM66268-LWLT should be assigned to the genus Streptomyces. On the basis of evidence from polyphasic study, strain TRM66268-LWLT is designated as representing a novel species of the genus Streptomyces, for which the name Streptomyces polyasparticus sp. nov. is proposed. The type strain is TRM66268-LWLT (CCTCC AA 2020003T = LMG32106T).

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Acknowledgment

Our research was supported by Science and Technology Research Project of Xin Jiang Production and Construction Corps (2018BC005), the National Natural Science Foundation of China (32060023), Microbial Resources Utilization Innovation Team in the Key Field of Xin Jiang Production and Construction Corps (2017CB014), the National Natural Science Foundation of China (U1703236) and the Postgraduate Student Research and Innovation Project of Xinjiang Uygur Autonomous Region (XJ2020G272).

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Authors and Affiliations

  1. Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, College of Life Science, Tarim University, Alar, 843300, Xinjiang, People’s Republic of China

    Wenlong Liu, Hong Zeng, Zhanfeng Xia, Xiaoxia Luo, Lili Zhang & Chuanxing Wan

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  1. Wenlong Liu
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  2. Hong Zeng
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  3. Zhanfeng Xia
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  4. Xiaoxia Luo
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Contributions

WLL contributed to performing the experiments, the morphological analyzes, genome analysis and writing the initial draft. CXW contributed to the guidance of experimental operations, reagents, instrumentation and the financial support for this work. LLZ and HZ contributed to the guidance of experimental operations. ZFX contributed to the morphological analyzes. XXL performed genome analysis. All authors approved the manuscript.

Corresponding authors

Correspondence to Wenlong Liu or Chuanxing Wan.

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This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.

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The GenBank/EMBL/DDBJ accession number for the genome and 16S rRNA gene sequence of strain TRM 66268-LWLT are JACTVJ000000000 and MT611106, respectively.

The SRA accession number for the genome of strain TRM66268-LWLT is PRJNA659572.

Supplementary Information

Fig. S1 Synthetic route of PASP

Fig. S2 Maximum-parsimony tree based on nearly complete 16S rRNA gene sequences showing relationships between strain TRM 66268-LWLT and the type strains of phylogenetically close Streptomyces species. Numbers at nodes were percentage bootstrap values based on 1000 resampled data sets; only values above 40% were given

Fig. S3 Maximum-likelihood tree based on nearly complete 16S rRNA gene sequences showing relationships between strain TRM 66268-LWLT and the type strains of phylogenetically close Streptomyces species. Numbers at nodes were percentage bootstrap values based on 1000 resampled data sets. Only values above 40% were given. Bar, 0.01 substitutions per nucleotide position

Fig. S4 Polar lipids of strain TRM 66268-LWLT separated by two-dimensional TLC and detected with anisaldehyde reagent (a), ninhydrin reagent (b) and molybdophosphoric acid reagent (c). The polar lipids detected diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylinositol mannoside (PIM) and two undetermined polar lipids L1-L2

Fig. S5 The strain of TRM66268-LWLT grown on the solid medium with PASP as the only source of carbon and nitrogen (A) and the only carbon source (B) at 28 °C for 1 month

Fig. S6 The physiology and pathway of PASP degradation.

Fig. S7 The PASP samples were buried in cotton field soil, and then the fermentation broth of the TRM66268-LWLT strain in Gauze’s No.1 medium for 7 days was applied to the soil. Dug out the samples one month later, SEM was used to observe the apparent morphology changes of the PASP samples (A, PASP standard sample; B, PASP sample was buried in cotton field for one month without adding fermentation broth of TRM66268-LWLT strain; C, PASP sample was buried in cotton field for one month and adding fermentation broth of TRM66268-LWLT strain).

Table S1 Characteristics of strain TRM 66268-LWLT grown on various media at 28 °C for 7 days.

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Liu, W., Zeng, H., Xia, Z. et al. Streptomyces polyasparticus sp. nov. isolated from cotton field soil by a medium applied with polyaspartic acid. Antonie van Leeuwenhoek 114, 777–786 (2021). https://doi.org/10.1007/s10482-021-01557-z

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