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Xylanimonas oleitrophica sp. nov., a novel petroleum hydrocarbon degrading bacterium isolated from an Indian oil reservoir

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Abstract

A novel Gram-stain-positive, aerobic, non-spore-forming, and rod-shaped bacterium designated as PW21was isolated from produced water sampled from an oil reservoir in Limbodara, Gujarat, India. Growth occurred at 30–45 °C (optimum 37 °C), at pH 6.5–9.5 (optimum pH 8.5) and in the presence of 0.5–5.0% (w/v) NaCl [optimum, 3% (w/v)]. Phylogenetic analysis based on the 16S rRNA gene sequences showed that PW21T belonged to the class Actinobacteria, order Actinomycetales, family Promicromonosporaceae, and genus Xylanimonas, with the highest sequence similarity to Isoptericola cucumis AP38 (97.95% with 98.8% completeness), followed by Xylanimonas pachnodae NBRC 107,786T (97.82%) and Xylanimonas allomyrinae 2JSPR-7T(97.75%) with 100% completeness for the mentioned strains of Xylanimonas. The genome size of PW21T was 3.4 Mbp with a G+C content of 73.0 mol% (draft genome sequence). The average nucleotide identity (ANI) value of the draft genomes between PW21T and related species were found in between 76.99 and 78.93%. Major cellular fatty acids (> 10% of total fatty acids) of PW21Twere iso-C15:0, anteiso-C15:0, and C16:0. Phylogenetic, physiological, and biochemical characterisation identified strain PW21T (= KCTC 49,338T = JCM 33,795T = MCC 3936T) as a novel species of the genus Xylanimonas, and hence, name Xylanimonas oleitrophica sp. nov. PW21T is proposed.

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Acknowledgements

Gratefully acknowledged: (1) Financial assistance from Agharkar Research Institute, Pune, India, and (2) University Grants Commission (UGC) for providing UGC-SRF to Pradnya Nagkirti by Government of India.

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Authors and Affiliations

  1. Bioenergy Group, MACS Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, India

    Pradnya D. Nagkirti, Anupama S. Engineer & Prashant K. Dhakephalkar

  2. Savitribai Phule Pune University, Ganeshkhind, Pune, 411007, India

    Pradnya D. Nagkirti & Prashant K. Dhakephalkar

Authors
  1. Pradnya D. Nagkirti
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  2. Anupama S. Engineer
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  3. Prashant K. Dhakephalkar
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Contributions

PDN performed experiments and wrote the manuscript. ASE designed the experiments and did the analysis. PKD designed the experiments, performed data validation and review the manuscript.

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Correspondence to Anupama S. Engineer or Prashant K. Dhakephalkar.

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Nagkirti, P.D., Engineer, A.S. & Dhakephalkar, P.K. Xylanimonas oleitrophica sp. nov., a novel petroleum hydrocarbon degrading bacterium isolated from an Indian oil reservoir. Antonie van Leeuwenhoek 114, 129–136 (2021). https://doi.org/10.1007/s10482-020-01506-2

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