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Streptomyces buecherae sp. nov., an actinomycete isolated from multiple bat species

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Abstract

A putative novel clade within the genus Streptomyces was discovered following antifungal screening against Pseudogymnoascus destructans, the causative agent of white-nose syndrome, and described using multi-locus sequencing analysis. Swabs from both the cave myotis bat (Myotis velifer) and the Brazilian free-tailed bat (Tadarida brasiliensis) in southern New Mexico bore isolates AC536, AC541T and AC563, which were characterised using phylogenetic, morphological, and phenotypic analyses. Multi-locus sequence analysis positions AC541T with neighbors Streptomyces rubidus (NRRL B-24619T), Streptomyces guanduensis (NRRL B-24617T), and Streptomyces yeochonensis (NRRL B-24245T). A complete genome of the type strain was assembled to determine its taxonomy and secondary metabolite potential. ANI comparisons between all closely related types strains are shown to be well below the 95–96% species delineation. DNA-DNA relatedness between AC541T and its nearest neighbors ranged between 23.7 and 24.1% confirming novelty. Approximately 1.49 Mb or 17.76% of the whole genome is devoted to natural product biosynthesis. The DNA G + C content of the genomic DNA of the type strain is 73.13 mol %. Micromorphology depicts ovoid spores with smooth surfaces in flexuous chains. Strains presented an ivory to yellow hue on most ISP media except inorganic salts-starch agar (ISP4) and can grow on d-glucose, mannitol, and d-fructose, but exhibited little to no growth on l-arabinose, sucrose, d-xylose, inositol, l-rhamnose, d-raffinose, and cellulose. This clade possesses the capability to grow from 10 to 45 °C and 12.5% (w/v) NaCl. There was strain growth variation in pH, but all isolates thrive at alkaline levels. Based on our polyphasic study of AC541T, the strain warrants the assignment to a novel species, for which the name Streptomyces buecherae sp. nov. is proposed. The type strain is AC541T (= JCM 34263T, = ATCC TSD201T).

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Acknowledgements

PSH and APA thank support from Western Illinois University and their Research Inspiring Student Excellence (RISE) and Women in Science (WIS) programs. CAD was supported by ARS National Programs 303, 304 and 306. DEN gratefully acknowledges the support of the staff at Carlsbad Caverns National Park with logistical support and assistance with the NPS Materials Transfer Agreement implementation. We would like to thank Dr. Ernest W. Valdez for providing expertise on bats. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture or the National Science Foundation. The mention of firm names or trade products does not imply that they are endorsed or recommended by the USDA over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer.

Funding

Initial funding was provided by the Eppley Foundation (DEN). Additional funding was provided by the IDNR (APA) and the Colorado Plateau Cooperative Ecosystem Studies Unit (CPCESU) (DEN). The lead author (PSH) would like to thank the Mycological Society of America for funding to present her research. This publication was also supported by the National Cancer Institute (NCI) of the National Institutes of Health (NIH) under Award Number F32CA221327 (MWM) and the National Center for Complementary and Integrative Health (NCCIH) of the NIH under Award Number R01AT009143 (RJT, NLK). This material is based upon work supported by the National Science Foundation (APA).

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Author notes

  1. Andrea Porras-Alfaro

    Present address: Division of Environmental Biology, National Science Foundation, Alexandria, VA, 22314, USA

Authors and Affiliations

  1. Department of Biological Sciences, Western Illinois University, Macomb, IL, 61455, USA

    Paris S. Hamm

  2. Department of Biology, University of New Mexico, Albuquerque, NM, 87131, USA

    Paris S. Hamm, Nicole A. Caimi & Diana E. Northup

  3. U. S. Department of Agriculture, Peoria, IL, 61604, USA

    Christopher A. Dunlap

  4. Department of Chemistry, Northwestern University, Evanston, IL, 60208, USA

    Michael W. Mullowney, Neil L. Kelleher & Regan J. Thomson

  5. Institute for Environmental Studies, Western Illinois University, Macomb, IL, 61455, USA

    Andrea Porras-Alfaro

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Contributions

PSH: Wrote the paper, Collected phenotypic and morphological data. CAD: Wrote the paper, performed genomic and phylogenetic analysis. MWM: Wrote the paper, Performed genomic and BGC analysis. NAC: Collected cultures. NLK: Obtained funding. RJT: Obtained funding. AP-A: Obtained funding, Reviewed/edited manuscript. DEN: Obtained funding, Collected the bats and cultures, Reviewed/edited manuscript.

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Correspondence to Paris S. Hamm.

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The GenBank accession number for complete genome of Streptomyces buecherae AC541T is CP060404.

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Hamm, P.S., Dunlap, C.A., Mullowney, M.W. et al. Streptomyces buecherae sp. nov., an actinomycete isolated from multiple bat species. Antonie van Leeuwenhoek 113, 2213–2221 (2020). https://doi.org/10.1007/s10482-020-01493-4

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