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Stenotrophomonas cyclobalanopsidis sp. nov., isolated from the leaf spot disease of Cyclobalanopsis patelliformis

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Abstract

A Gram-negative, facultatively anaerobic, motile bacterial strain, TPQG1-4T, was isolated from the leaf of Cyclobalanopsis patelliformis with spot disease. The isolate was investigated using the polyphasic taxonomic approach. 16S rRNA gene sequencing and analyzing revealed that the novel strain shares the highest sequence similarity with Stenotrophomonas lactitubi M15T (99.6%), Stenotrophomonas indicatrix WS40T (99.4%), Stenotrophomonas maltophilia IAM 12423T (99.2%) and Stenotrophomonas pavanii LMG 25348T (99.0%). In phylogenetic trees based on 16S rRNA gene sequences, the novel strain branched independently from other species of Stenotrophomonas. Average nucleotide identity values between the novel isolate and S. lactitubi M15T, S. indicatrix WS40T, S. maltophilia IAM 12423T, S. pavanii LMG 25348T, and Pseudomonas geniculata ATCC 19374T were 87.2%, 87.3%, 86.3%, 88.0%, and 81.3%, respectively, suggesting the isolate was a novel species of the genus Stenotrophomonas. The DNA G + C content of TPQG1-4T is 67.1 mol%. The major fatty acids were iso-C15:0 (25.4%) and anteiso-C15:0 (17.0%). The polar lipids of TPQG1-4T included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, amino phospholipid and phospholipid. Based on phenotypic and genotypic characteristics, the strain represents a novel species in the genus Stenotrophomonas, for which the name Stenotrophomonas cyclobalanopsidis sp. nov. is proposed. The type strain is TPQG1-4T (= CFCC 15341T = LMG 31208T).

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References

  • Baker GC, Simth JJ, Cowan DA (2003) Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55:541–555

    Article  CAS  Google Scholar 

  • Denton M, Kerr KG (1998) Microbiological and clinical aspects of infection associated with Stenotrophomonas maltophilia. Clin Microbiol Rev 11:57–80

    Article  CAS  Google Scholar 

  • Gerhardt P, Costilow RN, Krieg RGE, Nester EW, Phillips GB, Wood WA (1981) Manual of methods for general bacteriology. American Society for Microbiology, Washington, DC

    Google Scholar 

  • Goris J, Konstantindis KT, Klappenbach JA, Coenye T, Vandamme P, Tiedje JM (2007) DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57:81–91

    Article  CAS  Google Scholar 

  • Handa Y, Tazato N, Nagatsuka Y, Koide T, Kigawa R, Sano C, Sugiyama J (2016) Stenotrophomonas tumulicola sp. nov., a major contaminant of the stone chamber interior in the Takamatsuzuka Tumulus. Int J Syst Evol Microbiol 66:1119–1124

    Article  CAS  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  Google Scholar 

  • Lane DJ (1991) 16S/23S rRNA Sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematic. Wiley, New York, pp 115–175

    Google Scholar 

  • Lee Y, Jeon CO (2017) Cohnella algarum sp. nov., isolated from a freshwater green alga Paulinella chromatophora. Int J Syst Evol Microbiol 67:4767–4772

    Article  CAS  Google Scholar 

  • Li Y, Song LM, Guo MW, Wang LF, Liang WX (2016) Sphingobacterium populi sp. nov., isolated from bark of Populus × euramericana. Int J Syst Evol Microbiol 66:3456–3462

    Article  CAS  Google Scholar 

  • Li Y, Xue H, Guo LM, Koltay A, Palacio-Bielsa A, Chang J, Xie S, Yang X (2017) Elevation of three subspecies of Lonsdalea quercina to species level: Lonsdalea britannica sp. nov., Lonsdalea iberica sp. nov. and Lonsdalea populi sp. nov. Int J Syst Evol Microbiol 67:4680–4684

    Article  CAS  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Evol Microbiol 39:159–167

    CAS  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Ouattara AS, Le Mer J, Joseph M, Macarie H (2017) Transfer of Pseudomonas pictorum Gray and Thornton 1928 to genus Stenotrophomonas as Stenotrophomonas pictorum comb. nov., and emended description of the genus Stenotrophomonas. Int J Syst Evol Microbiol 67:1894–1900

    Article  CAS  Google Scholar 

  • Palleroni NJ, Bradbury JF (1993) Stenotrophomonas, a new bacterial genus for Xanthomonas maltophilia (Hugh 1980) Swings et al. 1983. Int J Syst Bacteriol 43:606–609

    Article  CAS  Google Scholar 

  • Ramos PL, Van Trappen S, Thompson FL, Rocha RC, Barbosa HR, De Vos P, Moreira-Filho CA (2011) Screening for endophytic nitrogen-fixing bacteria in Brazilian sugar cane varieties used in organic farming and description of Stenotrophomonas pavanii sp. nov. Int J Syst Evol Microbiol 61:926–931

    Article  CAS  Google Scholar 

  • Richter M, Rosselló-Móra R (2009) Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 10:19126–19131

    Article  Google Scholar 

  • Ryan RP, Monchy S, Cardinale M, Taghavi S, Crossman L, Avison MB, Berg G, van der Lelie D, Dow JM (2009) Versatility and adaptation of bacteria from the genus Stenotrophomonas. Nat Rev Microbiol 7:514–525

    Article  CAS  Google Scholar 

  • Sánchez-Castro I, Ruiz-Fresneda MA, Bakkali M, Kämpfer P, Glaseser SP, Busse HJ, López-Fernández M, Marítnez-Rodríguez P, Merroun ML (2017) Stenotrophomonas bentonitica sp. nov., isolated from bentonite formations. Int J Syst Evol Microbiol 67:2779–2786

    Article  Google Scholar 

  • Sasser M (2006) Bacterial identification by gas chromatographic analysis of fatty acids methyl estters (GC-FAME). MIDI Inc, Newark

    Google Scholar 

  • Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  CAS  Google Scholar 

  • Weber M, Schünemann W, Fuß J, Kämpfer P, Lipski A (2018) Stenotrophomonas lactitubi sp. nov. and Stenotrophomonas indicatrix sp. nov., isolated from surfaces with food contact. Int J Syst Evol Microbiol 68:1830–1838

    Article  CAS  Google Scholar 

  • Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613–1617

    Article  CAS  Google Scholar 

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Acknowledgements

The authors wish to acknowledge the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2018ZB001), and the National Infrastructure of Microbial Resources (NIMR-2019-7) from Ministry of Science and Technology of the People’s Republic of China.

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Authors and Affiliations

  1. The Key Laboratory of National Forestry and Grassland Administration on Forest Protection, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, China

    Dan-ran Bian, Han Xue, Chun-gen Piao & Yong Li

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  1. Dan-ran Bian
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  2. Han Xue
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  3. Chun-gen Piao
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  4. Yong Li
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Contributions

DRB wrote the manuscript and performed the research. HX performed the genomic analysis. CP conceived of this study. YL contributed to study design and revised the paper. All authors read and approved the final version of the manuscript.

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Correspondence to Yong Li.

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Bian, Dr., Xue, H., Piao, Cg. et al. Stenotrophomonas cyclobalanopsidis sp. nov., isolated from the leaf spot disease of Cyclobalanopsis patelliformis. Antonie van Leeuwenhoek 113, 1447–1454 (2020). https://doi.org/10.1007/s10482-020-01453-y

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